|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Virus, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the N-terminal region of the human and mouse Pen-2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Presenilins, which are components of the gamma-secretase protein complex, are required for intramembranous processing of some type I transmembrane proteins, such as the Notch proteins and the beta-amyloid precursor protein. Signaling by Notch receptors mediates a wide range of developmental cell fates. Processing of the beta-amyloid precursor protein generates neurotoxic amyloid beta peptides, the major component of senile plaques associated with Alzheimer's disease. This gene encodes a protein that is required for Notch pathway signaling, and for the activity and accumulation of gamma-secretase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
TRPC6 specifically interacts with APP to inhibit its cleavage by ¿-secretase and reduce Aß production.
36-7100 was used in western blot to characterize the interaction of TRPC6 with APP to inhibit its cleavage by gamma-secretase and reduce A-beta production
|Wang J,Lu R,Yang J,Li H,He Z,Jing N,Wang X,Wang Y||Nature communications (6:null)||2015|
Partial loss of presenilin impairs age-dependent neuronal survival in the cerebral cortex.
36-7100 was used in western blot to assess the effects of presenilin dosage on cortical neuron survival in mice.
|Watanabe H,Iqbal M,Zheng J,Wines-Samuelson M,Shen J||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:15912)||2014|
|Mouse||Not Cited||Trans-dominant negative effects of pathogenic PSEN1 mutations on ¿-secretase activity and Aß production.||Heilig EA,Gutti U,Tai T,Shen J,Kelleher RJ||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:11606)||2013|
|Mouse||Not Cited||Membrane-microdomain localization of amyloid ß-precursor protein (APP) C-terminal fragments is regulated by phosphorylation of the cytoplasmic Thr668 residue.||Matsushima T,Saito Y,Elliott JI,Iijima-Ando K,Nishimura M,Kimura N,Hata S,Yamamoto T,Nakaya T,Suzuki T||The Journal of biological chemistry (287:19715)||2012|
Familial frontotemporal dementia-associated presenilin-1 c.548G>T mutation causes decreased mRNA expression and reduced presenilin function in knock-in mice.
36-7100 was used in western blot to study the effects of a familial frontotemporal dementia-associated presenilin- c.548G>T mutation in knock-in mice.
|Watanabe H,Xia D,Kanekiyo T,Kelleher RJ,Shen J||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:5085)||2012|
|Mouse||1:200||Involvement of 5-lipoxygenase in the corticosteroid-dependent amyloid beta formation: in vitro and in vivo evidence.||Puccio S,Chu J,Praticò D||PloS one (6:null)||2011|
|Mouse||1:200||5-lipoxygenase as an endogenous modulator of amyloid ß formation in vivo.||Chu J,Praticò D||Annals of neurology (69:34)||2011|
|Transcriptional regulation of PEN-2, a key component of the gamma-secretase complex, by CREB.||Wang R,Zhang YW,Sun P,Liu R,Zhang X,Zhang X,Xia K,Xia J,Xu H,Zhang Z||Molecular and cellular biology (26:1347)||2006|
||Trans-dominant negative effects of pathogenic PSEN1 mutations on ¿-secretase activity and Aß production.||Heilig EA,Gutti U,Tai T,Shen J,Kelleher RJ||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:11606)||2013|