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Detection of an oligohistidine fusion protein with the Penta·His mouse monoclonal antibody. Twofold serial dilutions of an Escherichia coli lysate containing overexpressed oligomycin sensitivity–conferring protein (OSCP) fused with a hexahistidine domain were run an SDS-polyacrylamide gel and blotted onto a PVDF membrane. The blot was stained with the SYPRO Ruby protein blot stain (S11791) to detect the entire protein profile (top). After imaging, the blot was incubated with Penta·His mouse IgG<sub>1</sub> monoclonal antibody (P21315), followed by immunodetection using an alkaline phosphatase conjugate of goat anti–mouse antibody in conjunction with DDAO phosphate (bottom).
|Tested species reactivity||Tag|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Histidine Tag (5xHis)|
|Storage buffer||PBS with PEG, sucrose|
|Contains||0.08% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
5Xhis; Histidine 5X; pentahistidine