|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Recombinant fragment corresponding to residues 98-534 of mouse Perforin.|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 ug/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25830 detects Perforin from mouse samples.
MA1-25830 has been successfully used in immunocytochemistry, immunohistochemistry (frozen), immunohistochemistry (paraffin), immunoprecipitation, and Western blot procedures. Positve control of mouse T cell clone B6.1 suggested.
The MA1-25830 immunogen is a recombinant fragment corresponding to residues 98-534 of mouse Perforin.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Perforin is one of the major cytolytic proteins of cytolyic granules. One of the main pathways of lymphocyte mediated cytolysis entails the secretion onto target membranes of cytolytic granules contained in cytolytic effector lymphocytes of T-cell or NK-cell type. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Perforin (PRF) is involved in the killing mediated by cytotoxic lymphocytes which represents an important mechanism of transmembrane channel formation similar to C9 and homology between perforin and C9 has been demostrated at their respective functionally conserved regions. Studies show that perforin is expressed only in killer cell lines and not in helper T lymophocytes or other tumor cells tested.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.