|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant fragment corresponding to residues 98-534 of mouse Perforin.|
|Purification||Size-exclusion - Gel filtration|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25835 detects Perforin from human and mouse samples.
MA1-25835 has been successfully used in Western blot and immunoprecipitation procedures. MA1-25835 detects a band ~C66 kDa from the mouse T cell clone B6.1 (pos. control). This antibody does not detect human perforin by western blot.
The MA1-25835 immunogen is a recombinant fragment corresponding to residues 98-534 of mouse Perforin.
Perforin is one of the major cytolytic proteins of cytolytic granules. One of the main pathways of lymphocyte mediated cytolysis entails the secretion onto target membranes of cytolytic granules contained in cytolytic effector lymphocytes of T-cell or NK-cell type. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Perforin (PRF) is involved in the killing mediated by cytotoxic lymphocytes which represents an important mechanism in the immune defense against tumors and virus infections. Human PRF is a 555 amino acid protein with a 21 amino acid signal peptide. It has a molecular weight of 70 to 75 kD. PRF is a pore forming protein with a mechanism of transmembrane channel formation similar to C9 and homology between perforin and C9 has been demonstrated at their respective functionally conserved regions. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.