|Tested species reactivity||Bovine, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide made to a region of the Human OXPAT protein, within residues 300-400.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 30% glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1.0-5.0 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1.0-5 µg/ml|
|Western Blot (WB)||0.5-2.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The target sequence has 94% sequence homology with sheep and porcine and 84% sequence homology with rat.
Suggested positive control: human and mouse heart lysate.
OXPAT is a novel PAT-1 protein that is expressed in highly oxidative tissues. Lipid droplet proteins of the PAT family regulate cellular neutral lipid stores. OXPAT is induced by physiological (fasting), pathophysiological (insulin deficiency), pharmacological (peroxisome proliferator-activated receptor [PPAR] agonists), and genetic (muscle-specific PPAR alpha overexpression) disturbances that increase fatty acid utilization. It is suggested that OXPAT is a marker for PPAR activation and fatty acid oxidation. As well, it may contribute to adaptive responses to the fatty acid responsibility that accompanies fasting, insulin deficiency, and overnutrition, responses that are defective in obesity and type 2 diabetes
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.