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|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) S M N K G P T L L D G D L P E Q(17) C of rat Perilipin A and B.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
PA1-1052 detects perilipin A and B from rat and 3T3-L1 cell extract.
PA1-1052 has been successfully used in Western blot procedures. By Western blot, this antibody detects ~62 and 46 kDa proteins representing perilipin A and B, respectively from 3T3-L1 cell extract
The PA1-1052 immunogen is a synthetic peptide corresponding to residues M(1) S M N K G P T L L D G D L P E Q(17) C of rat Perilipin A and B. This sequence is 82% conserved in humans. The PA1-1052 immunizing peptide (Cat. # PEP-170) is available for use in neutralization and control experiments.
Adipose tissue is an energy reserve in animals and is strictly regulated in nondomestic species. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), resistin, and perilipin. Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, perilipin is phosphorylated by protein kinase A. Once activated, perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body.
Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Isolation of lipid droplets from cells by density gradient centrifugation.
PA1-1052 was used in western blot to evaluate a density gradient centrifugation protocol for the isolation of cellular lipid droplets
|Brasaemle DL,Wolins NE||Current protocols in cell biology (Chapter 3:null)||2006|
Translocation of hormone-sensitive lipase and perilipin upon lipolytic stimulation of rat adipocytes.
PA1-1052 was used in western blot to investigate the influence of adipocyte lipolysis on the distribution of HSL and perilipin as well as the phosphorylation of perilipin
|Clifford GM,Londos C,Kraemer FB,Vernon RG,Yeaman SJ||The Journal of biological chemistry (275:5011)||2000|
ADFP; Adipophilin; adipose differentiation related protein; Adipose differentiation-related protein; ADRP; Lipid droplet-associated protein; PERI; PERIA; Perilipin A; perilipin B; Perilipin-1; Perilipin-2; PLIN; Plin1; Plin2
6030432J05Rik; AA407157; Adfp; ADPH; Adrp; Peri; PERIA; Plin; Plin1; Plin2