Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunohistochemistry analysis of PERLECAN showing staining in the cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a PERLECAN Mouse Monoclonal Antibody (134400) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Primate, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||rec-Perlecan domain III.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Perlecan is a major heparan-sulfate proteoglycan (HSPG) found within all basement membranes and cell surfaces. Because of its strategic location and ability to store and protect growth factors, perlecan has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Remodeling of extracellular matrix by normal and tumor-associated fibroblasts promotes cervical cancer progression.
13-4400 was used in western blot to study the role of normal and tumor-associated fibroblasts in the pathogenesis of cervical cancer.
|Fullár A,Dudás J,Oláh L,Hollósi P,Papp Z,Sobel G,Karászi K,Paku S,Baghy K,Kovalszky I||BMC cancer (15:null)||2015|
|Human||Not Cited||Confluence switch signaling regulates ECM composition and the plasmin proteolytic cascade in keratinocytes.||Botta A,Delteil F,Mettouchi A,Vieira A,Estrach S,Négroni L,Stefani C,Lemichez E,Meneguzzi G,Gagnoux-Palacios L||Journal of cell science (125:4241)||2012|
|Human||2 µg/ml||Primary human glomerular endothelial cells produce proteoglycans, and puromycin affects their posttranslational modification.||Björnson A,Moses J,Ingemansson A,Haraldsson B,Sörensson J||American journal of physiology. Renal physiology (288:F748)||2005|
Peripheral nerve hyperexcitability with preterminal nerve and neuromuscular junction remodeling is a hallmark of Schwartz-Jampel syndrome.
13-4400 was used in immunohistochemistry to characterize nerve hyperexcitability and neuromuscular junction remodeling in Schwartz-Jampel syndrome
|Bauché S,Boerio D,Davoine CS,Bernard V,Stum M,Bureau C,Fardeau M,Romero NB,Fontaine B,Koenig J,Hantaï D,Gueguen A,Fournier E,Eymard B,Nicole S||Neuromuscular disorders : NMD (23:998)||2013|
|Primate||Not Cited||Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane zone.||Evans MJ,Fanucchi MV,Van Winkle LS,Baker GL,Murphy AE,Nishio SJ,Sannes PL,Plopper CG||American journal of physiology. Lung cellular and molecular physiology (283:L1263)||2002|
||Confluence switch signaling regulates ECM composition and the plasmin proteolytic cascade in keratinocytes.||Botta A,Delteil F,Mettouchi A,Vieira A,Estrach S,Négroni L,Stefani C,Lemichez E,Meneguzzi G,Gagnoux-Palacios L||Journal of cell science (125:4241)||2012|
|Human||Not Cited||Pericyte recruitment during vasculogenic tube assembly stimulates endothelial basement membrane matrix formation.||Stratman AN,Malotte KM,Mahan RD,Davis MJ,Davis GE||Blood (114:5091)||2009|
|Primate||1:2000||Atypical development of the tracheal basement membrane zone of infant rhesus monkeys exposed to ozone and allergen.||Evans MJ,Fanucchi MV,Baker GL,Van Winkle LS,Pantle LM,Nishio SJ,Schelegle ES,Gershwin LJ,Miller LA,Hyde DM,Sannes PL,Plopper CG||American journal of physiology. Lung cellular and molecular physiology (285:L931)||2003|
basement membrane-specific heparan sulfate proteoglycan core protein; endorepellin (domain V region); perlecan proteoglycan
HSPG; HSPG2; PLC; PRCAN; SJA; SJS; SJS1