|Tested species reactivity||Bovine, Human, Mouse, Pig|
|Published species reactivity||Bovine, Mouse, Human|
|Host / Isotype||Rat / IgG2a, kappa|
|Immunogen||Murine EHS laminin preparation|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1-2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 5 publications below|
MA5-14638 targets Perlecan in IF and IHC (P, F) applications and shows reactivity with Bovine, Human, mouse, and Porcine samples.
The MA5-14638 immunogen is mouse EHS laminin preparation.
Perlecan is a major heparan-sulfate proteoglycan (HSPG) found within all basement membranes and cell surfaces. Because of its strategic location and ability to store and protect growth factors, perlecan has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Liver aging and pseudocapillarization in a Werner syndrome mouse model.
MA5-14638 was used in immunohistochemistry to study the accelerated age-related hepatic changes observed in a murine model of Werner syndrome
|Cogger VC,Svistounov D,Warren A,Zykova S,Melvin RG,Solon-Biet SM,O'Reilly JN,McMahon AC,Ballard JW,De Cabo R,Le Couteur DG,Lebel M||The journals of gerontology. Series A, Biological sciences and medical sciences (69:1076)||2014|
LARGE glycans on dystroglycan function as a tunable matrix scaffold to prevent dystrophy.
MA5-14638 was used in immunohistochemistry to study the role of dystroglycan LARGE glycans in the normal functioning of skeletal muscle
|Goddeeris MM,Wu B,Venzke D,Yoshida-Moriguchi T,Saito F,Matsumura K,Moore SA,Campbell KP||Nature (503:136)||2013|
DOT1L regulates dystrophin expression and is critical for cardiac function.
MA5-14638 was used in immunohistochemistry to study the role of DOT1L in regulating dystrophin expression and cardiac function
|Nguyen AT,Xiao B,Neppl RL,Kallin EM,Li J,Chen T,Wang DZ,Xiao X,Zhang Y||Genes and development (25:263)||2011|
The effect of bovine endosteum-derived particles on the proliferation of human mesenchymal stem cells.
MA5-14638 was used in immunohistochemistry to evaluate the usefulness of bovine endosteum-derived particles in stem cell culture
|Nigro J,White JF,Ramshaw JA,Haylock DN,Nilsson SK,Werkmeister JA||Biomaterials (31:5689)||2010|
Synthesis of syndecan-1 by skeletal muscle cells is an early response to infection with Trichinella spiralis but is not essential for nurse cell development.
MA5-14638 was used in immunohistochemistry to study the synthesis of syndecan-1 by skeletal muscle cells in response to Trichinella spiralis infection
|Beiting DP,Park PW,Appleton JA||Infection and immunity (74:1941)||2006|