Immunohistochemistry analysis of Perlecan showing staining in the cytoplasm (Basement membrane cell) of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Perlecan Rat Monoclonal Antibody (MA514641) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Bovine, Human, Mouse, Pig|
|Published species reactivity||Bovine, Mouse, Human, Not Applicable|
|Host / Isotype||Rat / IgG2a, kappa|
|Immunogen||Murine EHS laminin preparation|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:20|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14641 targets Perlecan in IF, IHC (F), and IHC (P, F) applications and shows reactivity with Bovine, Human, mouse, and Porcine samples.
The MA5-14641 immunogen is mouse EHS laminin preparation.
Perlecan is a major heparan-sulfate proteoglycan (HSPG) found within all basement membranes and cell surfaces. Because of its strategic location and ability to store and protect growth factors, perlecan has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Liver aging and pseudocapillarization in a Werner syndrome mouse model.
MA5-14641 was used in immunohistochemistry to study the accelerated age-related hepatic changes observed in a murine model of Werner syndrome
|Cogger VC,Svistounov D,Warren A,Zykova S,Melvin RG,Solon-Biet SM,O'Reilly JN,McMahon AC,Ballard JW,De Cabo R,Le Couteur DG,Lebel M||The journals of gerontology. Series A, Biological sciences and medical sciences (69:1076)||2014|
LARGE glycans on dystroglycan function as a tunable matrix scaffold to prevent dystrophy.
MA5-14641 was used in immunohistochemistry to study the role of dystroglycan LARGE glycans in the normal functioning of skeletal muscle
|Goddeeris MM,Wu B,Venzke D,Yoshida-Moriguchi T,Saito F,Matsumura K,Moore SA,Campbell KP||Nature (503:136)||2013|
DOT1L regulates dystrophin expression and is critical for cardiac function.
MA5-14641 was used in immunohistochemistry to study the role of DOT1L in regulating dystrophin expression and cardiac function
|Nguyen AT,Xiao B,Neppl RL,Kallin EM,Li J,Chen T,Wang DZ,Xiao X,Zhang Y||Genes and development (25:263)||2011|
The effect of bovine endosteum-derived particles on the proliferation of human mesenchymal stem cells.
MA5-14641 was used in immunohistochemistry to evaluate the usefulness of bovine endosteum-derived particles in stem cell culture
|Nigro J,White JF,Ramshaw JA,Haylock DN,Nilsson SK,Werkmeister JA||Biomaterials (31:5689)||2010|
Synthesis of syndecan-1 by skeletal muscle cells is an early response to infection with Trichinella spiralis but is not essential for nurse cell development.
MA5-14641 was used in immunohistochemistry to study the synthesis of syndecan-1 by skeletal muscle cells in response to Trichinella spiralis infection
|Beiting DP,Park PW,Appleton JA||Infection and immunity (74:1941)||2006|
|Not Applicable||Not Cited||
Anti-DNA autoantibodies initiate experimental lupus nephritis by binding directly to the glomerular basement membrane in mice.
MA5-14641 was used in immunohistochemistry - frozen section to test if direct binding of anti-DNA antibody to the glomerular basement membrane important in experimental lupus nephritis
|Krishnan MR,Wang C,Marion TN||Kidney international (82:184)||2012|
Heparan sulfate proteoglycans as regulators of fibroblast growth factor-2 signaling in brain endothelial cells. Specific role for glypican-1 in glioma angiogenesis.
MA5-14641 was used in western blot to investigate the influence of heparan sulfate proteoglycans on fibroblast growth factor-2 signal pathway in brain endothelial cells
|Qiao D,Meyer K,Mundhenke C,Drew SA,Friedl A||The Journal of biological chemistry (278:16045)||2003|