Immunofluorescence analysis of Peroxiredoxin 1 was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PRDX1 Rabbit Polyclonal (PA3750) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytosolic and nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues L(103) V S D P K R T I A Q D(114) of human Peroxiredoxin 1.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1,000 - 1:5,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
PA3-750 detects Peroxiredoxin 1 protein in human, rat and mouse samples. This antibody is specific for Prx 1 and shows no cross-reactivity with other Prx isoforms.
PA3-750 has successfully been used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~26 kDa protein representing Prx 1 in human PC3 cell lysate.
The PA3-750 immunizing peptide corresponds to amino acid residues 103-114 from human Prx 1.
The peroxiredoxin (Prdx, Prx, or Trx-Px) family of enzymes is a recently identified family of peroxidases found in free-living organisms. The six known isoforms (Prx1-6) play an important role in protecting lipids, enzymes and DNA against peroxides, such as hydrogen peroxide. The ubiquitously expressed peroxiredoxins have also been shown to play a role in apoptosis and cell differentiation. This is acomplished by the active cysteine of Prx reducing peroxides, which is then converted into a transient cysteine sulfenic acid or cysteine sulfinic acid. If Prx protection is inadequate against peroxidases, the resulting protein and DNA damage may result in neurological disease such as Alzheimer and quote;s or DNA damage leading to cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Proteomic analysis of the effects of aged garlic extract and its FruArg component on lipopolysaccharide-induced neuroinflammatory response in microglial cells.
PA3-750 was used in western blot to study the effects of aged garlic extract and its FruArg component by proteomic analysis and lipopolysaccharide-induced neuroinflammatory response in microglial cells
|Zhou H,Qu Z,Mossine VV,Nknolise DL,Li J,Chen Z,Cheng J,Greenlief CM,Mawhinney TP,Brown PN,Fritsche KL,Hannink M,Lubahn DB,Sun GY,Gu Z||PloS one (9:null)||2014|
Proteomic profiling identifies cyclooxygenase-2-independent global proteomic changes by celecoxib in colorectal cancer cells.
PA3-750 was used in western blot to find new celecoxib-mediated markers in the COX-2-deficient colorectal cancer cell line
|Lou J,Fatima N,Xiao Z,Stauffer S,Smythers G,Greenwald P,Ali IU||Cancer epidemiology, biomarkers and prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology (15:1598)||2006|
Nonredundant antioxidant defense by multiple two-cysteine peroxiredoxins in human prostate cancer cells.
PA3-750 was used in western blot to investigate the roles of Prxs in antioxidant defense in human prostate cancer cells
|Shen C,Nathan C||Molecular medicine (Cambridge, Mass.) (8:95)||2002|