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|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues L(103) V S D P K R T I A Q D(114) of human Peroxiredoxin 1.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
PA3-750 detects Peroxiredoxin 1 protein in human, rat and mouse samples. This antibody is specific for Prx 1 and shows no cross-reactivity with other Prx isoforms.
PA3-750 has successfully been used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~26 kDa protein representing Prx 1 in human PC3 cell lysate.
The PA3-750 immunizing peptide corresponds to amino acid residues 103-114 from human Prx 1.
The peroxiredoxin (Prdx, Prx, or Trx-Px) family of enzymes is a recently identified family of peroxidases found in free-living organisms. The six known isoforms (Prx1-6) play an important role in protecting lipids, enzymes and DNA against peroxides, such as hydrogen peroxide. The ubiquitously expressed peroxiredoxins have also been shown to play a role in apoptosis and cell differentiation. This is acomplished by the active cysteine of Prx reducing peroxides, which is then converted into a transient cysteine sulfenic acid or cysteine sulfinic acid. If Prx protection is inadequate against peroxidases, the resulting protein and DNA damage may result in neurological disease such as Alzheimer"e;s or DNA damage leading to cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Proteomic profiling identifies cyclooxygenase-2-independent global proteomic changes by celecoxib in colorectal cancer cells.
PA3-750 was used in western blot to find new celecoxib-mediated markers in the COX-2-deficient colorectal cancer cell line
|Lou J,Fatima N,Xiao Z,Stauffer S,Smythers G,Greenwald P,Ali IU||Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology (15:1598)||2006|
Nonredundant antioxidant defense by multiple two-cysteine peroxiredoxins in human prostate cancer cells.
PA3-750 was used in western blot to investigate the roles of Prxs in antioxidant defense in human prostate cancer cells
|Shen C,Nathan C||Molecular medicine (Cambridge, Mass.) (8:95)||2002|
heme-binding 23 kDa protein; macrophage 23 Kd stress protein; macrophage 23 kDa stress protein; macrophage 23kDa stress protein; macrophase stress protein 22kDa; macrophase stress protein 23 kd; natural killer cell-enhancing factor A; natural killer-enhancing factor A; osteoblast specific factor 3; osteoblast-specific factor 3; peroxiredoxin-1; proliferation-associated gene A; proliferation-associated gene protein; Prx 1; thioredoxin dependent peroxide reductase 2; thioredoxin peroxidase 2; thioredoxin-dependent peroxide reductase 2; Trx dependent peroxide reductase 2
Hbp23; MSP23; NKEF-A; NKEFA; OSF-3; OSF3; PAG; PAGA; PAGB; PRDX1; PrdxI; PRX1; PRXI; TDPX2; TDX2; TPxA