|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues L(104) L A D V T R R L S E D(115) of human Peroxiredoxin 2.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA3-751 detects Peroxiredoxin 2 protein in human samples. This antibody is specific for Prx 2 and shows no cross-reactivity with other Prx isoforms.
PA3-751 has successfully been used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~26 kDa protein representing Prx 2 in human PC3 cell lysate.
The PA3-751 immunizing peptide corresponds to amino acid residues 104-115 from human Prx 2.
The peroxiredoxin (Prdx, Prx, or Trx-Px) family of enzymes is a recently identified family of peroxidases found in free-living organisms. The six known isoforms (Prx1-6) play an important role in protecting lipids, enzymes and DNA against peroxides, such as hydrogen peroxide. The ubiquitously expressed peroxiredoxins have also been shown to play a role in apoptosis and cell differentiation. This is acomplished by the active cysteine of Prx reducing peroxides, which is then converted into a transient cysteine sulfenic acid or cysteine sulfinic acid. If Prx protection is inadequate against peroxidases, the resulting protein and DNA damage may result in neurological disease such as Alzheimer and quote;s or DNA damage leading to cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Nonredundant antioxidant defense by multiple two-cysteine peroxiredoxins in human prostate cancer cells.
PA3-751 was used in western blot to investigate the roles of Prxs in antioxidant defense in human prostate cancer cells
|Shen C,Nathan C||Molecular medicine (Cambridge, Mass.) (8:95)||2002|