|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||1:20|
|Immunocytochemistry (ICC)||20 µl/ml|
|Immunoprecipitation (IP)||1-2 µl|
|Western Blot (WB)||1:500 - 1:2000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, recombinant, human fragment protein (without mitochondrial leader sequence) expressed in E.coli.|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
A suggested positive control for this product is HeLa cells.
Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20-30 kilodalton in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Alu co-repressor 1; Alu corepressor 1; Antioxidant enzyme B166; AOEB166; epididymis secretory protein Li 55; Liver tissue 2D-page spot 2D-0014IV; Liver tissue 2D-page spot 71B; peroxiredoxin 6; Peroxiredoxin V; Peroxiredoxin-5, mitochondrial; Peroxisomal antioxidant enzyme; peroxisomal membrane protein 20; PLP; Prx-V; Prx5; Thioredoxin peroxidase PMP20; Thioredoxin reductase; TPx type VI
Gene Aliases: ACR1; AOEB166; AOPP; B166; HEL-S-55; PLP; PMP20; PRDX5; PRDX6; prx-V; PRXV; SBBI10
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