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Localization of phosphatidylinositol 3,4,5-triphosphate in unstimulated (upper panel) and insulin-stimulated (lower panel) 3T3-L1 cells detected using anti–phosphatidylinositol 3,4,5-triphosphate antibdy (mouse IgM monoclonal RC6F8, A21328) and visualized using biotinylated goat anti–mouse IgM antibody in conjunction with Alexa Fluor 488 streptavidin (S11223). Image courtesy of Echelon Research Laboratories, Inc.
|Tested species reactivity||Human , Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgM|
|Storage buffer||PBS, pH 7.2, with 0.1% BSA|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
Recent research has revealed direct actions of phosphatidylinositol 4,5-biphosphate (PtdIns(4,5)P2) and phosphatidylinositol 3,4,5-triphosphate (PtdIns(3,4,5)P3) on a diverse array of cellular functions including actin assembly and cytoskeletal dynamics, vesicular protein trafficking, protein kinase localization and activation, cell proliferation and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
BCR-ABL inactivates cytosolic PTEN through Casein Kinase II mediated tail phosphorylation.
A-21328 was used in immunocytochemistry to demonstrate that nuclear exclusion of PTEN is associated with PTEN inactivation in the cytoplasm of CML cells.
|Morotti A,Panuzzo C,Crivellaro S,Carrà G,Fava C,Guerrasio A,Pandolfi PP,Saglio G||Cell cycle (Georgetown, Tex.) (14:973)||2015|
Phosphatidylinositol (3, 4, 5)-trisphosphate, PIP3, Phosphatidylinositol 3, 5-triphosphate