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|Tested species reactivity||Mouse , Human|
|Published species reactivity||Human , Not Applicable|
|Host / Isotype||Mouse / IgM|
|Storage buffer||PBS, pH 7.2, with 0.1% BSA|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Phosphatidylinositol 4,5-disphosphate or PIP2 is an important second messenger for a number of signaling processes. When certain extracellular signals are received, Phosphilipase C cleaves phosphatidylinositol into DAG and PIP2. PIP2 has been shown to interact with actin structural proteins including profiling, gelsolin, and cofilin, and demonstrates regulatory function of actin polymerization. It has also been shown to regulate vesicular transport by interacting with proteins like ADP-ribosylation factor and phospholipase D. PIP2 has also been shown to significantly affect intracellular calcium concentrations, which also regulate a variety of activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cell susceptibility to baculovirus transduction and echovirus infection is modified by protein kinase C phosphorylation and vimentin organization.
A-21327 was used in immunocytochemistry to understand the effect of protein kinase C phosphorylation on cell susceptibility to baculovirus transduction and echovirus infection
|Turkki P,Makkonen KE,Huttunen M,Laakkonen JP,Ylä-Herttuala S,Airenne KJ,Marjomäki V||Journal of virology (87:9822)||2013|
|Not Applicable||Not Cited||
Diacylglycerol stimulates acrosomal exocytosis by feeding into a PKC- and PLD1-dependent positive loop that continuously supplies phosphatidylinositol 4,5-bisphosphate.
A-21327 was used in western blot to elucidate how diacylglycerol promotes acrosomal exocytosis.
|Lopez CI,Pelletán LE,Suhaiman L,De Blas GA,Vitale N,Mayorga LS,Belmonte SA||Biochimica et biophysica acta (1821:1186)||2012|
Phosphatidylinositol 4, 5-diphosphate, PtdInsP2, PIP2