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|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||Liposomes (prepared in PBS with lipid A, phosphatidylcholine (PC) and cholesterol) containing synthetic di-palmitoyl PtdIns(4,5)P2.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-500 detects PIP2 from human tissues.
MA3-500 has been successfully used in ELISA, Immunoprecipitation, Immunofluorescence, and Western blotting procedures. By Western blot it detects a 67-kDa band representing MA3-500.
The MA3-500 antigen is liposomes containing synthetic di-palmitoyl ptdlns(4,5)P2.
Phosphatidylinositol 4,5-bisphosphate or PIP2 is an important second messenger for a number of signaling processes. When certain extracellular signals are received, Phosphilipase C cleaves phosphatidylinositol into DAG and PIP2. PIP2 has been shown to interact with actin structural proteins including profiling, gelsolin, and cofilin, and demonstrates regulatory function of actin polymerization. It has also been shown to regulate vesicular transport by interacting with proteins like ADP-ribosylation factor and phospholipase D. PIP2 has also been shown to significantly affect intracellular calcium concentrations, which also regulate a variety of activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Phosphatidylinositol Diphosphate; PIP 2