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Western blot of rat brainstem lysate showing specific immunolabeling of the ~29 kDa 14-3-3 protein phosphorylated at Ser58 (Lane 1). The labeling by Anti-Ser58 14-3-3 is specifically blocked by the Ser58 phosphopeptide (Lane 2) used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling (not shown). For a more detailed version ofFigure 1, please visit our website.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 of rat YWHAB conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
This antibody is predicted to react with bovine, canine, chicken, mouse, non-human primate, sheep, Xenopus and zebrafish based on 100% sequence homology.
This antibody is specific for the ~29 kDa 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by preadsorption of the antibody with the protein used to generate the antibody.
14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, its dimeric structure. Recent work as demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation (Woodcock et al., 2003).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CCM3/PDCD10 stabilizes GCKIII proteins to promote Golgi assembly and cell orientation.
PA1-4612 was used in western blot to investigate the effect of CCM3 on the regulation of centrosome and Golgi and its mechanism
|Fidalgo M,Fraile M,Pires A,Force T,Pombo C,Zalvide J||Journal of cell science (123:1274)||2010|
Serine 58 of 14-3-3zeta is a molecular switch regulating ASK1 and oxidant stress-induced cell death.
PA1-4612 was used in western blot to investigate the regulation of phosphorylation status of serine 58 in 14-3-3 and its relationship with cell death caused by oxidative stress
|Zhou J,Shao Z,Kerkela R,Ichijo H,Muslin AJ,Pombo C,Force T||Molecular and cellular biology (29:4167)||2009|
14-3-3 alpha; 14-3-3 protein beta-subtype; 14-3-3 protein beta/alpha; 3-monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide; brain protein 14-3-3, beta isoform; epididymis secretory protein Li 1; KCIP 1; KCIP-1; prepronerve growth factor RNH-1; protein 1054; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, beta polypeptide
GW128; HEL-S-1; HS1; KCIP-1; YWHAA; YWHAB