|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser523 of human ALOX5 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
This antibody is predicted to react with non-human primate based on 100% sequence homology.
This antibody is specific for the ~80 kDa 5-LO phosphorylated at Ser523 in Western blots of rat hippocampal lysates.
The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LT ) (Funk, 2001). Overproduction of LT contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-pecific antibody to Ser523 on 5-LO is thus likely to provide a valuable toll for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Na+/H+ exchanger regulatory factor 1 overexpression-dependent increase of cytoskeleton organization is fundamental in the rescue of F508del cystic fibrosis transmembrane conductance regulator in human airway CFBE41o- cells.
PA1-4628 was used in western blot to study the effect of sodium/hydrogen exchanger regulatory factor 1 on F508del cystic fibrosis transmembrane conductance regulator
|Favia M,Guerra L,Fanelli T,Cardone RA,Monterisi S,Di Sole F,Castellani S,Chen M,Seidler U,Reshkin SJ,Conese M,Casavola V||Molecular biology of the cell (21:73)||2010|
5-Lipoxygenase in the Prefrontal Cortex of Suicide Victims.
PA1-4628 was used in western blot to study the protein level of 5-Lipoxygenase in suicide victim brains and its role in depression
|Uz T,Dwivedi Y,Pandey GN,Roberts RC,Conley RR,Manev R,Manev H||The open neuropsychopharmacology journal (1:1)||2008|