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Western blot analysis of Phospho-A-Raf pTyr302 using Phospho-A-Raf pTyr302 polyclonal antibody (Product # PA5-36783) at a dilution of 1:500. Lane 1: Hela cell lysate treated with PMA (100nM, 15min), Lane 2: sp2/0 cell lysate treated with PMA (100nM, 15min), Lane 3: PC12 cell lysate treated with PMA (100nM, 15min).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human A-Raf around the phosphorylation site of Tyrosine 302|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 68 kDa.
Purity is >95% by SDS-PAGE.
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A-Raf proto-oncogene serine/threonine-protein kinase; ARAF; ARAF1; Oncogene ARAF1; PKS; PKS2; proto-oncogene A-Raf; proto-oncogene A-Raf-1; proto-oncogene Pks; Raf related protein; raf-related oncogene; Ras-binding protein; Ras-binding protein DA-Raf; serine/threonine-protein kinase A-Raf; v-raf murine sarcoma 3611 viral oncogene homolog 1; v-raf murine sarcoma 3611 viral oncogene-like protein; v-raf oncogene homolog 1 (murine sarcoma 3611 virus)
1200013E08Rik; A-RAF; ARAF; ARAF1; AW495444; PKS; PKS2; RAFA1