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|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of tyrosine 284 (D-H-Y(p)-V-M) derived from Human ACK1.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for Western blot is HepG2 cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is A549 cells.
This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ACK; ACK1; activated CDC42 kinase 1; activated Cdc42-associated kinase 1; activated p21cdc42Hs kinase; Cdc42 GTPase-inhibiting protein; non-receptor protein tyrosine kinase ACK; p21cdc42Hs; P21cdc42Hs kinase; tyrosine kinase non-receptor protein 2; tyrosine kinase, non-receptor, 2
ACK; ACK-1; ACK1; Cdgip; p21cdc42Hs; Pyk1; TNK2