Invitrogen

Phospho-ADD1/ADD2 (Ser726, Ser713) Polyclonal Antibody

Product Image Gallery

Phospho-ADD1/ADD2 (Ser726, Ser713) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-ADD1/ADD2 (Ser726, Ser713) in paraffin-embedded human rectum carcinoma tissue (membrane and cytoplasmic staining) and negative control (right, with PBS only). Samples were incubated with Phospho-ADD1/ADD2 (Ser726, Ser713) polyclonal antibody (Product # PA5-36614) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.

Phospho-ADD1/ADD2 (Ser726, Ser713) Antibody in Western Blot (WB) — Western blot analysis of Phospho-ADD1/ADD2 (Ser726, Ser713) in Lane 1: HeLa whole cell lysate (40 µg), Lane 2: HeLa treated with PMA (60 ng/mL, 30 min) whole cell lysate (40 µg), Lane 3: PC12 whole cell lysate (40 µg), Lane 4: PC12 treated with PMA (60 ng/mL, 30 min) whole cell lysate (40 µg). Samples were incubated with Phospho-ADD1/ADD2 (Ser726, Ser713) polyclonal antibody (Product # PA5-36614) at a dilution of 1:500.

Phospho-ADD1/ADD2 (Ser726, Ser713) Antibody in Immunohistochemistry (IHC) — FIGURE 7 Immunohistochemistry of selected phosphorylated proteins in the EC at different stages of NFT pathology (stages I-II, III-IV, and V-VI of Braak). Phosphorylated catenin-beta and p38-P immunoreactivity appear at the first and middle stages of NFT pathology as small granules in the cytoplasm of a subpopulation of neurons. This pattern is also found for p38-P in many neurons of the EC at stages V-VI of NFT pathology. MAP2-P, NFL-P, and SAPK/JNK-P immunoreactivity is found in neurons with the morphology of NFTs from the first stages onwards; the number of affected neurons increases with disease progression. The number of NFL-P-positive neurons is, by far, smaller than the number of neurons with MAP2-P pathology in consecutive sections. PAK1-P immunoreactivity is seen in NFTs at stages III-IV and V-VI, but as irregular or granular deposits in EC neurons at staged I-II. ADD1-P immunoreactivity increases in astrocytes and in a subpopulation of NFTs at middle and advanced stages of NFT pathology. Paraffin sections, lightly counterstained with hematoxylin, bar = 50 mum

Product Details

PA5-36614

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic phosphopeptide derived from human Adducin alpha around the phosphorylation site of Serine 726
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2553614

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 81 kDa.

Purity is >95% by SDS-PAGE.

Target Information

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dysregulated protein phosphorylation: A determining condition in the continuum of brain aging and Alzheimer's disease.

Brain pathology (Zurich, Switzerland)

Ferrer I,Andrés-Benito P,Ausín K,Pamplona R,Del Rio JA,Fernández-Irigoyen J,Santamaría E

Published figure using Phospho-ADD1/ADD2 (Ser726, Ser713) polyclonal antibody (Product # PA5-36614) in Immunohistochemistry

Mon Nov 01 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: Add97; adducin 1 (alpha); adducin 2 (beta); adducin beta; Adducin-1; Adducin-63; Adducin-alpha; Adducin-beta; Adducin1; alpha Adducin; alpha-ADD; Alpha-adducin; beta Adducin; Beta-adducin; erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha; Erythrocyte adducin subunit beta; snoRNA MBII-396

Gene Aliases: 2900072M03Rik; ADD1; ADD2; add97; ADDA; ADDB; AI256389; beta-ADD

UniProt ID: (Human) P35611, (Human) P35612, (Mouse) Q9QYC0, (Rat) Q63028, (Mouse) Q9QYB8, (Rat) Q05764

Entrez Gene ID: (Human) 118, (Human) 119, (Mouse) 11518, (Rat) 24170, (Mouse) 11519, (Rat) 24171

Molecular Function: non-motor actin binding protein