Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
|Tested species reactivity||Mouse , Human|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antibody was produced against a chemically synthesized phosphopeptide derived from the region of human Akt that contains serine 473.|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||1:200-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 6 publications below|
This product contains enough material for 10 mini-blots.
The immunogenic sequence is conserved among multiple species including human, mouse and rat in all three isoforms: Akt1, Akt2 and Akt3.
Recommended positive controls include NIH-3T3 cells stimulated with PDGF and 3T3-L1 adipocytes stimulated with OSM and IFN-gamma.
AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A high-fish-oil diet prevents adiposity and modulates white adipose tissue inflammation pathways in mice.
44-621G was used in western blot to study the regulatory effect of a high-fish-oil diet on rodent white adipose tissue inflammation pathways
|Bargut TC,Mandarim-de-Lacerda CA,Aguila MB||The Journal of nutritional biochemistry (26:960)||2015|
Ganitumab (AMG 479) inhibits IGF-II-dependent ovarian cancer growth and potentiates platinum-based chemotherapy.
44-621G was used in western blot to assess the therapeutic potential of ganitumab for the treatment of ovarian cancer.
|Beltran PJ,Calzone FJ,Mitchell P,Chung YA,Cajulis E,Moody G,Belmontes B,Li CM,Vonderfecht S,Velculescu VE,Yang G,Qi J,Slamon DJ,Konecny GE||Clinical cancer research : an official journal of the American Association for Cancer Research (20:2947)||2014|
PLC-γ and PI3K link cytokines to ERK activation in hematopoietic cells with normal and oncogenic Kras.
44-621G was used in western blot to demonstrate that phospholipase C-γ, PI3K, and their generated second messengers link activated cytokine receptors to Ras and ERK signaling in differentiated bone marrow cells and leukemia stem cells.
|Diaz-Flores E,Goldschmidt H,Depeille P,Ng V,Akutagawa J,Krisman K,Crone M,Burgess MR,Williams O,Houseman B,Shokat K,Sampath D,Bollag G,Roose JP,Braun BS,Shannon K||Science signaling (6:null)||2013|
Noncovalent wild-type-sparing inhibitors of EGFR T790M.
44-621G was used in western blot to identify indolocarbazole compounds as potentially effective EGFR inhibitors.
|Lee HJ,Schaefer G,Heffron TP,Shao L,Ye X,Sideris S,Malek S,Chan E,Merchant M,La H,Ubhayakar S,Yauch RL,Pirazzoli V,Politi K,Settleman J||Cancer discovery (3:168)||2013|
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
44-621G was used in western blot to study the effect of receptor tyrosine kinases ligands on cancer resistance.
|Wilson TR,Fridlyand J,Yan Y,Penuel E,Burton L,Chan E,Peng J,Lin E,Wang Y,Sosman J,Ribas A,Li J,Moffat J,Sutherlin DP,Koeppen H,Merchant M,Neve R,Settleman J||Nature (487:505)||2012|
Neuregulin-1-mediated autocrine signaling underlies sensitivity to HER2 kinase inhibitors in a subset of human cancers.
44-621G was used in western blot to suggest that lapatinib may benefit patients with NRG-driven tumors lacking HER2 amplification.
|Wilson TR,Lee DY,Berry L,Shames DS,Settleman J||Cancer cell (20:158)||2011|
PKB-ALPHA, PKB/Akt, PKB, Rac, CWS6, AKT, PKBalpha, RAC-ALPHA, PRKBA, RAC, Akt
PKB alpha, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase, protein kinase B alpha, proto-oncogene c-Akt, rac protein kinase alpha, AKT1 kinase, Akt1m protein, protein kinase B-alpha, proto-oncogene c-AKT, related to A and C kinases, AKT, C-AKT, EC 22.214.171.124, PKB, PKB-alpha, RAC, RAC-alpha serine/threonine kinase, kinase Akt1