This Antibody was verified by Cell Treatment to ensure that the antibody binds to the antigen stated. View Details
This antibody is predicted to react with Human, Monkey, Rat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
AKT1 (RAC-alpha serine/threonine-protein kinase) is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2, AKT3) which regulate many processes including metabolism, proliferation, cell survival, growth, and angiogenesis. It's mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them no isoform specificity has been reported. AKT is responsible for the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at Ser-50 negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT also regulates the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. In addition, AKT regulates cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development, and synapse formation. Mutations in the gene can result in breast cancer, colorectal cancer, Proteus syndrome, and Cowden syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AKT1 kinase; AKT1m; Akt1m protein; PKB; PKB alpha; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; related to A and C kinases; serine-threonine protein kinase; Thymoma viral proto-oncogene; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1; RAC.
Gene Aliases: AKT; AKT1; CWS6; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA
Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase