This Antibody was verified by Cell Treatment to ensure that the antibody binds to the antigen stated. View Details
OMA1-03061 detects protein kinase B (PKB) phosphorylated at Ser473 from human and mouse samples. OMA1-03061 detects PKB 1,2,and 3.
OMA1-03061 has been successfully used in Western blot, immunofluorescence, and immunocytochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing phosphorylated PKB (Ser473) from NIH-3T3 cells treated with platelet-derived growth factor (PDGF).
The OMA1-03061 immunizing phosphopeptide was derived from a region of human PKB that contains serine 473. There is 100% homology with other species, that have not yet been tested.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
Protein kinase B (PKB), also known as Akt, is an oncogenic serine-threonine kinase originally cloned from the retrovirus AKT8. PKB is a direct target of phosphatidylinositol 3-kinase (PI3-kinase) and is activated in response to a variety of growth factors including insulin and platelet-derived growth factor (PDGF). PKB also plays an important role in many PI3-kinase dependent cellular functions including inhibition of apoptosis.
Activity of PKB has been associated with the phosphorylation of two sites: T308, in the activation loop of the kinase, and S473, at the carboxyl terminus. Phosphorylation of both sites contributes to PKB activity, however phosphorylation of T308 has been shown to be absolutely essential for PKB activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AKT1 kinase; AKT1m; Akt1m protein; Phospho-AKT; Phospho-PKB; PKB; PKB alpha; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; related to A and C kinases; serine-threonine protein kinase; Thymoma viral proto-oncogene; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
Gene Aliases: AKT; AKT1; CWS6; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA
Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase