Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1-3 µg/mL | View 6 publications 6 publications |
Immunohistochemistry (IHC) |
- | View 2 publications 2 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
0.5-2 µg/mL | View 2 publications 2 publications |
Immunocytochemistry (ICC/IF) |
1-2 µg | - |
Flow Cytometry (Flow) |
1-3 µg/1x10^6 cells | View 3 publications 3 publications |
ELISA (ELISA) |
1-5 µg/mL | - |
Functional Assay (FN) |
- | View 1 publication 1 publication |
Miscellaneous PubMed (Misc) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse |
Published species |
Bacteria, Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Recombinant Monoclonal |
Type |
Antibody |
Clone |
98H9L8 |
Immunogen |
A peptide corresponding to amino acids 468-477 of P31749. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS |
Contains |
0.09% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2532320 |
This antibody is predicted to react with bovine, canine, chicken, chimpanzee, equine, feline, rat, Rhesus monkey , Xenopus and zebrafish based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AKT1 kinase; AKT1m; Akt1m protein; C-AKT; CAKT; PKB; PKB alpha; PKBG; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; related to A and C kinases; serine-threonine protein kinase; Thymoma viral proto-oncogene; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
Gene Aliases: AKT; AKT1; CWS6; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA
UniProt ID: (Human) P31749, (Mouse) P31750
Entrez Gene ID: (Human) 207, (Mouse) 11651
Molecular Function:
non-receptor serine/threonine protein kinase
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