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      Phospho-AKT2 (Ser474) Polyclonal Antibody

      See 11 other AKT2 antibodies ›
      Phospho-AKT2 (Ser474) Polyclonal Antibody for Western Blot and IHC (P)

      Catalog#: PA5-36819 Invitrogen

      Catalog # Size Price (USD) Qty Datasheet
      PA5-36819 100 µl
      349.00
      Please enter a quantity in the range of 1 to 199.
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      Product Details

      Bioinformatics

      Documents
      Details
      Tested species reactivity Human, Mouse, Rat
      Host / Isotype Rabbit / IgG
      Class Polyclonal
      Type Antibody
      Immunogen Synthetic phosphopeptide derived from human AKT2 around the phosphorylation site of Serine 474
      Conjugate Unconjugated
      Form Liquid
      Concentration 1 mg/ml
      Purification Antigen affinity chromatography
      Storage buffer PBS, pH 7.2
      Contains 0.05% sodium azide
      Storage Conditions Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
      Tested Applications Dilution *
      Immunohistochemistry (Paraffin) (IHC (P)) 1:50-1:200
      Western Blot (WB) 1:500-1:1000

      * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

      Product Specific Information

      This antibody detects endogenous protein at a molecular weight of 60 kDa. It does not cross-react with AKT1 and AKT3 protein when phosphorylated at the corresponding residues.

      Purity is >95% by SDS-PAGE.

      Background/Target Information

      AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      Bioinformatics

      Protein Aliases:

      AKT2; AKT2 kinase; murine thymoma viral (v-akt) homolog-2; murine thymoma viral (v-akt) oncogene homolog 2; PKB beta; Protein kinase Akt-2; Protein kinase B beta; protein kinase B, beta; putative v-akt murine thymoma viral oncoprotein 2; rac protein kinase beta; RAC protein kinase beta RAC-PK beta; RAC-beta serine/threonine protein kinase; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; thymoma viral proto-oncogene 2; v-akt murine thymoma viral oncogene homolog 2

      Gene Aliases:

      2410016A19Rik; AKT2; AW554154; HIHGHH; PKB; PKBB; PKBBETA; PRKBB; RAC-BETA

      UniProt ID:

      (Human) P31751, (Rat) P47197, (Mouse) Q60823

      Entrez Gene ID:

      (Human) 208, (Rat) 25233, (Mouse) 11652

      Molecular Function:

      annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase

      Function(s) Process(es)

      Documents

      Material safety data sheets (MSDS)

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