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Invitrogen

Phospho-AKT2 (Ser474) Polyclonal Antibody

View all (59) AKT2 antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

PA5-36819

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide derived from human AKT2 around the phosphorylation site of Serine 474
View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2553754

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 60 kDa. It does not cross-react with AKT1 and AKT3 protein when phosphorylated at the corresponding residues.

Purity is >95% by SDS-PAGE.

Target Information

AKT2 is a phosphoinositide-dependent serine/threonine protein kinase that is enriched in insulin responsive tissues and has been implicated in the metabolic actions of the hormone. Akt is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AKT2 kinase; murine thymoma viral (v-akt) homolog-2; murine thymoma viral (v-akt) oncogene homolog 2; PKB beta; Protein kinase Akt-2; Protein kinase B beta; protein kinase B, beta; putative v-akt murine thymoma viral oncoprotein 2; rac protein kinase beta; RAC protein kinase beta RAC-PK beta; RAC-beta serine/threonine protein kinase; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; thymoma viral proto-oncogene 2; v-akt murine thymoma viral oncogene homolog 2

Gene Aliases: 2410016A19Rik; AKT2; AW554154; HIHGHH; PKB; PKBB; PKBBETA; PRKBB; RAC-BETA

UniProt ID: (Human) P31751, (Rat) P47197, (Mouse) Q60823

Entrez Gene ID: (Human) 208, (Rat) 25233, (Mouse) 11652

Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme

Function(s)
positive regulation of protein phosphorylation glycogen biosynthetic process glucose metabolic process regulation of translation cellular protein modification process apoptotic process response to osmotic stress signal transduction insulin receptor signaling pathway carbohydrate transport positive regulation of signal transduction negative regulation of plasma membrane long-chain fatty acid transport positive regulation of glucose metabolic process response to muscle activity peptidyl-serine phosphorylation regulation of cell migration positive regulation of cell migration positive regulation of vesicle fusion response to nutrient levels positive regulation of fatty acid beta-oxidation peripheral nervous system myelin maintenance cellular response to insulin stimulus negative regulation of RNA splicing positive regulation of peptidyl-serine phosphorylation intracellular signal transduction negative regulation of cysteine-type endopeptidase activity involved in apoptotic process protein kinase B signaling positive regulation of nitric oxide biosynthetic process fat cell differentiation positive regulation of glycogen biosynthetic process positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import positive regulation of positive chemotaxis mammary gland epithelial cell differentiation intracellular protein transmembrane transport regulation of cell cycle arrest cellular response to organic cyclic compound protein localization to plasma membrane positive regulation of protein targeting to membrane activation of GTPase activity positive regulation of cell motility positive regulation of glucose import in response to insulin stimulus protein phosphorylation multicellular organism development positive regulation of gene expression positive regulation of sodium ion transport response to insulin cellular response to hormone stimulus negative regulation of apoptotic process carbohydrate metabolic process glycogen metabolic process transport phosphorylation
Process(es)
protein serine/threonine kinase activity protein kinase C binding protein binding ATP binding protein kinase activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
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