Western blot analysis of Phospho-AMPK-alpha (pThr172) was performed by loading 30ug of THP-1 cell lysates from cells either treated with a vehicle control (C, left lane) or with 100nM PMA (right lane) for 15 minutes, onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat dry milk in TBST for 1 hour at room temperature. The membrane was probed with a Phospho-AMPK-alpha pThr172 polyclonal antibody (Product # PA5-17831) at a dilution of 1:1000 for overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-rabbit IgG at a dilution of 1:40,000 for 1 hour at room temperature. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.
|Tested species reactivity||Bovine, Chicken, Human, Mouse, Non-human primate, Pig, Rat|
|Published species reactivity||Sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pThr172 of human AMPKa|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/ml BSA|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
It is not recommended to aliquot this antibody.
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Palmitoleic acid reduces intramuscular lipid and restores insulin sensitivity in obese sheep.
PA5-17831 was used in western blot to investigate the effects of palmitoleic acid on lipogenesis and circulating insulin levels in obese sheep
|Duckett SK,Volpi-Lagreca G,Alende M,Long NM||Diabetes, metabolic syndrome and obesity : targets and therapy (7:553)||2014|