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Western blot analysis of Phospho-AMPKbeta1 pSer182 in extracts from oligomycin-treated C6 cells or C6 cell lysate treated with lambda phosphatase, using Phospho-AMPKbeta1 pSer182 polyclonal antibody (Product # PA5-17513) (upper) or an AMPK-beta1 polyclonal antibody (lower).
|Tested species reactivity||Human, Mouse, Primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pSer182 of human AMPKß1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
5'-AMP-activated protein kinase 40 kDa subunit; 5'-AMP-activated protein kinase beta-1 subunit; 5'-AMP-activated protein kinase subunit beta-1; 5'-AMP-activated protein kinase, beta subunit; 5-AMP-activated protein kinase beta subunit; AMP-activated protein kinase beta subunit; AMPK beta -1 chain; AMPK beta 1; AMPK beta-1 chain; AMPK subunit beta-1; AMPKb; protein kinase, AMP-activated, beta 1 non-catalytic subunit; protein kinase, AMP-activated, noncatalytic, beta-1
1300015D22Rik; AMPK; AU021155; E430008F22; HAMPKb; PRKAB1