Invitrogen

Phospho-APLF (Ser116) Polyclonal Antibody

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Phospho-APLF (Ser116) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Phospho-APLF (Ser116) in Human breast tissue using a Phospho-APLF (Ser116) polyclonal antibody (Product # PA5-118677). The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

Phospho-APLF (Ser116) Antibody in Western Blot (WB) — Western blot analysis of Phospho-APLF (Ser116) in starvation treated K562 cells using a Phospho-APLF (Ser116) polyclonal antibody (Product # PA5-118677). The -/+ symbols identify the absence or presence of N peptide (non-phospho peptide) and P peptide(phospho peptide).

Product Details

PA5-118677

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human APLF around the phosphorylation site of Ser116. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2903177

Target Information

C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks.[supplied by OMIM, Mar 2008].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Aprataxin and PNK-like factor; aprataxin- and PNK-like factor; Apurinic-apyrimidinic endonuclease APLF; PNK and APTX-like FHA domain-containing protein; PNK and APTX-like FHA protein; XRCC1-interacting protein 1; zinc finger, CX5CX6HX5H motif containing 1

Gene Aliases: 2010301N04Rik; AI452191; APFL; APLF; C2orf13; PALF; RGD1565557; XIP1; ZCCHH1

UniProt ID: (Human) Q8IW19, (Mouse) Q9D842

Entrez Gene ID: (Human) 200558, (Rat) 500247, (Mouse) 72103

Function(s)

single strand break repair DNA catabolic process, endonucleolytic double-strand break repair cellular response to DNA damage stimulus biological_process regulation of isotype switching positive regulation of DNA ligation nucleic acid phosphodiester bond hydrolysis DNA repair

Process(es)

nucleotide binding DNA-(apurinic or apyrimidinic site) lyase activity endodeoxyribonuclease activity protein binding 3'-5' exonuclease activity metal ion binding molecular_function lyase activity

Phospho-APLF (Ser116) Polyclonal Antibody

Product Details

PA5-118677

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

References (0)

Bioinformatics

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