Western blot analysis of Phospho-AS160 pThr642 in whole cell extract (80ug per lane) from HeLa cells (serum-starved) treated with IGF1 (150 ng/mL, 15 min) using a Phospho-AS160 pThr642 recombinant rabbit monoclonal antibody (Product # 700565) at a dilution of 2.5ug/ml. Detection was performed using an HRP-conjugated goat anti-rabbit secondary antibody at a dilution of 1:5000 followed by chemiluminescence (ECL). Results show a band at ~160kDa.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide with the sequence RRRAH[pT]FSHPPS corresponding to amino acids 637–648 of AS160|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
AS160 domain family member 4], is a 160 kDa protein expressed in many tissues including brain, kidney, and liver. The protein is a Rab GTPase activating protein (GAP) involved in control of energy functions such as glucose and lipid metabolism. In humans, the gene is located on the q arm of chromosome 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.