|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunocytochemistry (ICC)||1 µg/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Western Blot (WB)||2-5 ug/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide with the sequence RRRAH[pT]FSHPPS corresponding to amino acids 637–648 of AS160|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with mouse based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
AS160 domain family member 4], is a 160 kDa protein expressed in many tissues including brain, kidney, and liver. The protein is a Rab GTPase activating protein (GAP) involved in control of energy functions such as glucose and lipid metabolism. In humans, the gene is located on the q arm of chromosome 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Acrg embryonic lethality minimal region ortholog; Akt substrate of 160 kDa; AS160; BUB2; CDC16) domain-containing protein; TBC (Tre-2; TBC (Tre-2, BUB2, CDC16) domain-containing protein; TBC1 domain family member 4; TBC1 domain family, member 4
Gene Aliases: AS160; KIAA0603; NIDDM5; TBC1D4
UniProt ID: (Human) O60343
Entrez Gene ID: (Human) 9882
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