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Invitrogen

Phospho-ATF2 (Thr69) Polyclonal Antibody

View all (73) ATF2 antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

PA5-36622

Applications Tested Dilution Publications

Immunohistochemistry (Paraffin) (IHC (P))

 1:50-1:200 -

Immunoprecipitation (IP)

 1:50-1:200 -
Product Specifications

Species Reactivity

 Human, Mouse, Rat

Host/Isotype

 Rabbit / IgG

Class

 Polyclonal

Type

 Antibody

Immunogen

Synthetic phosphopeptide derived from human ATF2 around the phosphorylation site of Threonine 69
View immunogen

Conjugate

 Unconjugated

Form

 Liquid

Concentration

 1 mg/mL

Purification

 Antigen affinity chromatography

Storage buffer

 PBS, pH 7.2, with 50% glycerol

Contains

 0.02% sodium azide

Storage conditions

 Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2553619

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 55-75 kDa.

Purity is >95% by SDS-PAGE.

Target Information

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Activating 2; Activating transcription factor 2; activating transcription factor 2 splice variant ATF2-var2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; CREB-2; Cyclic AMP-dependent transcription factor ATF-2; Cyclic AMP-responsive element-binding protein 2; HB16; histone acetyltransferase ATF2; MGC111558; MXBP protein; OTTHUMP00000205345; OTTHUMP00000205347; OTTHUMP00000205348; OTTHUMP00000205350; OTTHUMP00000205352; OTTHUMP00000205353; OTTHUMP00000205354

Gene Aliases: Atf-2; ATF2; CRE-BP; CRE-BP1; CREB-2; CREB2; CREBP1; D130078H02Rik; D18875; HB16; mXBP; Tg(Gzma-Klra1)7Wum; TREB7

UniProt ID: (Human) P15336, (Rat) Q00969, (Mouse) P16951

Entrez Gene ID: (Human) 1386, (Rat) 81647, (Mouse) 11909

Molecular Function: basic leucine zipper transcription factor

Function(s)
negative regulation of transcription from RNA polymerase II promoter outflow tract morphogenesis regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter response to osmotic stress cellular response to DNA damage stimulus response to water deprivation histone acetylation intra-S DNA damage checkpoint positive regulation of transforming growth factor beta2 production positive regulation of neuron apoptotic process fat cell differentiation positive regulation of transcription from RNA polymerase II promoter negative regulation of epithelial cell proliferation regulation of sequence-specific DNA binding transcription factor activity positive regulation of sequence-specific DNA binding transcription factor activity adipose tissue development amelogenesis positive regulation of mitochondrial membrane permeability involved in apoptotic process transcription, DNA-templated
Process(es)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity histone acetyltransferase activity protein binding cAMP response element binding protein binding protein kinase binding cAMP response element binding metal ion binding protein heterodimerization activity DNA binding sequence-specific DNA binding nucleic acid binding transferase activity
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