Invitrogen

Phospho-ATP1A1 (Tyr260) Polyclonal Antibody

View all (40) ATP1A1 antibodies

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Datasheet

Protocols

Questions & Answers

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Phospho-ATP1A1 (Tyr260) Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of Phospho-ATP1A1 (Tyr260) in 293 cells using a Phospho-ATP1A1 (Tyr260) polyclonal antibody (Product # PA5-118769). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1:600, was used as secondary antibody.

Phospho-ATP1A1 (Tyr260) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Phospho-ATP1A1 (Tyr260) in Mouse colorectal tissue using a Phospho-ATP1A1 (Tyr260) polyclonal antibody (Product # PA5-118769). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

Phospho-ATP1A1 (Tyr260) Antibody in Western Blot (WB) — Western blot analysis of Phospho-ATP1A1 (Tyr260) in HepG2 cells using a Phospho-ATP1A1 (Tyr260) polyclonal antibody (Product # PA5-118769). The -/+ symbols identify the absence or presence of N peptide (non-phospho peptide) and P peptide(phospho peptide).

Product Details

PA5-118769

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human ATP1A1 around the phosphorylation site of Tyr260. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2903269

Target Information

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP1B; ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+K+ transporting, alpha 1; MGC1798; Na(+)/K(+) ATPase alpha-1 subunit; Na+/K+ ATPase 1; Na+/K+ ATPase Alpha; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; RP4-655J12.1; Sodium Potassium ATPase; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide; Sodium/potassium-transporting ATPase subunit alpha-1

Gene Aliases: ATP1A1; Atpa-1; BC010319; Nkaa1b

UniProt ID: (Human) P05023, (Rat) P06685, (Mouse) Q8VDN2

Entrez Gene ID: (Human) 476, (Rat) 24211, (Mouse) 11928

Molecular Function: primary active transporter