Invitrogen

Phospho-ATR (Ser428) Polyclonal Antibody

View all (23) ATR antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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Phospho-ATR (Ser428) Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of Phospho-ATR (Ser428) in U2OS cells using a Phospho-ATR (Ser428) Polyclonal antibody (Product # PA5-121295). Blue: DAPI for nuclear staining.

Phospho-ATR (Ser428) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Phospho-ATR (Ser428) in human testis using a Polyclonal antibody (Product # PA5-121295) at a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..

Phospho-ATR (Ser428) Antibody in Western Blot (WB) — Western Blot analysis of Phospho-ATR (Ser428) in NIH/3T3 cells using a Phospho-ATR (Ser428) Polyclonal antibody (Product # PA5-121295) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 30s.

Product Details

PA5-121295

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500 - 1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50 - 1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic phosphorylated peptide around S428 of human ATR (NP_001175.2). View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.49 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.05% ProClin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2914867

Product Specific Information

Positive test controls include: NIH/3T3. The target is usually found in the following locations: Chromosome, Nucleus, PML body.

Target Information

The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 2310008J16Rik; 2810405N18Rik; ataxia telangiectasia and Rad3 related; Ataxia telangiectasia and Rad3-related protein; ATR serine/threonine kinase; EC 2.7.11.1; FRAP-related protein 1; FRAP-related protein-1; kinase ATR; MEC1, mitosis entry checkpoint 1, homolog; protein kinase ATR; Serine/threonine-protein kinase ATR; TEM8

Gene Aliases: ATR; FCTCS; FRP1; MEC1; SCKL; SCKL1

UniProt ID: (Human) Q13535

Entrez Gene ID: (Human) 545, (Mouse) 245000, (Rat) 685055

Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme