Western blot analysis of Phospho-ATRIP pSer239 in mouse testis lysate using a Phospho-ATRIP pSer239 polyclonal antibody (Product # PA5-35392). Results show a band at ~86kDa. Phosphospecificity is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30 min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser239 of human ATRIP conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 0.1mg/ml BSA, 0.15M NaCl, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, non-human primate and sheep based on 100% sequence homology.
This antibody contains enough material to conduct 10 mini-Western blots.
ATRIP is an 86 kDa protein that is phosphorylated by ATR, regulates ATR expression, and is an essential component of the DNA damage checkpoint pathway. It localizes to the intranuclear foci after DNA damage or inhibition of replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.