Western blot analysis of Phospho-Androgen Receptor pSer94 in HeLa lysate using a Phospho-Androgen Receptor pSer94 polyclonal antibody (Product # PA5-35386). Results show a band at ~110kDa. The phosphospecificity is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide used as antigen.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser94 of human AR conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 0.1mg/ml BSA, 0.15M NaCl, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with non-human primate based on 100% sequence homology.
This antibody contains enough material to conduct 10 mini-Western blots.
The androgen receptor (AR) is an ~110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. IGF-1 activates the phosphatidylinositol 3-kinase(PI3K)/AKT pathway in LNCap at high passage number and increases phosphorylation of of AR at Ser213/210 and Ser791/790. The western blot results also show that inhibition of the PI3K/Akt pathway by LY294002 prior to incubation with IGF-1 suppressed AR phosphorylation at Ser213/210. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.