Invitrogen

Phospho-Annexin A1 (Tyr21) Polyclonal Antibody

View all (28) Annexin A1 antibodies

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Phospho-Annexin A1 (Tyr21) Antibody in Western Blot (WB) — Western blot analysis of Phospho-Annexin A1 (Tyr21) in Jurkat cell lysate (absence (-) or presence (+) of non-phospho and phospho peptide). Samples were incubated with Phospho-Annexin A1 (Tyr21) polyclonal antibody (Product # PA5-105965).

Phospho-Annexin A1 (Tyr21) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Phospho-Annexin A1 (Tyr21) in rat lung tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Annexin A1 (Tyr21) polyclonal antibody (Product # PA5-105965) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-Annexin A1 (Tyr21) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Phospho-Annexin A1 (Tyr21) in human kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Annexin A1 (Tyr21) polyclonal antibody (Product # PA5-105965) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-Annexin A1 (Tyr21) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Phospho-Annexin A1 (Tyr21) in mouse brain tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Annexin A1 (Tyr21) polyclonal antibody (Product # PA5-105965) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000-1:3000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ANXA1 around the phosphorylation site of Tyr21.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 150mM NaCl

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2817364

Target Information

Annexin I belongs to a family of Ca (2+) -dependent phospholipid binding proteins that are preferentially located on the cytosolic face of the plasma membrane. Annexin I has a molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Annexin 1 (p35) (Lipocortin 1); Annexin A1; Annexin I; annexin I (lipocortin I); Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; lipocortin 1; Lipocortin I; p35; Phospholipase A2 inhibitory protein; RP11-71A24.1

Gene Aliases: Anx-1; Anx-A1; ANX1; ANXA1; C430014K04Rik; Lpc-1; LPC1; p35

UniProt ID: (Human) P04083, (Mouse) P10107, (Rat) P07150

Entrez Gene ID: (Human) 301, (Mouse) 16952, (Rat) 25380

DNA strand renaturation neutrophil homeostasis signal transduction cell surface receptor signaling pathway peptide cross-linking insulin secretion keratinocyte differentiation positive regulation of vesicle fusion positive regulation of prostaglandin biosynthetic process DNA duplex unwinding positive regulation of neutrophil apoptotic process DNA rewinding regulation of cell proliferation positive regulation of apoptotic process alpha-beta T cell differentiation arachidonic acid secretion negative regulation of protein secretion cellular response to hydrogen peroxide prolactin secretion cellular response to glucocorticoid stimulus neutrophil clearance positive regulation of G1/S transition of mitotic cell cycle negative regulation of phospholipase A2 activity negative regulation of interleukin-8 secretion response to hormone response to X-ray response to organic cyclic compound prostate gland development endocrine pancreas development response to corticosteroid response to estradiol gliogenesis response to drug response to peptide hormone estrous cycle response to glucocorticoid hepatocyte differentiation response to interleukin-1 adaptive immune response monocyte chemotaxis regulation of leukocyte migration phagocytosis inflammatory response G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger regulation of cell shape myoblast migration involved in skeletal muscle regeneration actin cytoskeleton reorganization regulation of interleukin-1 production positive regulation of interleukin-2 production positive regulation of T cell proliferation negative regulation of apoptotic process innate immune response positive regulation of T-helper 1 cell differentiation negative regulation of T-helper 2 cell differentiation negative regulation of exocytosis regulation of hormone secretion regulation of inflammatory response granulocyte chemotaxis positive regulation of wound healing

Process(es)

single-stranded DNA binding single-stranded RNA binding helicase activity receptor binding structural molecule activity calcium ion binding protein binding phospholipid binding calcium-dependent phospholipid binding phospholipase A2 inhibitor activity protein binding, bridging double-stranded DNA-dependent ATPase activity annealing helicase activity protein homodimerization activity calcium-dependent protein binding cadherin binding involved in cell-cell adhesion phospholipase inhibitor activity