Invitrogen

Phospho-Annexin A1 (Tyr21) Polyclonal Antibody

View all (70) Annexin A1 antibodies

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Phospho-Annexin A1 (Tyr21) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Phospho-Annexin A1 (Tyr21) in human kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Annexin A1 (Tyr21) polyclonal antibody (Product # PA5-105965) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-Annexin A1 (Tyr21) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Phospho-Annexin A1 (Tyr21) in mouse brain tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Annexin A1 (Tyr21) polyclonal antibody (Product # PA5-105965) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-Annexin A1 (Tyr21) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Phospho-Annexin A1 (Tyr21) in rat lung tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Annexin A1 (Tyr21) polyclonal antibody (Product # PA5-105965) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-Annexin A1 (Tyr21) Antibody in Western Blot (WB) — Western blot analysis of Phospho-Annexin A1 (Tyr21) in Jurkat cell lysate (absence (-) or presence (+) of non-phospho and phospho peptide). Samples were incubated with Phospho-Annexin A1 (Tyr21) polyclonal antibody (Product # PA5-105965).

Product Details

PA5-105965

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ANXA1 around the phosphorylation site of Tyr21.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2817364

Target Information

Annexin I belongs to a family of Ca (2+) -dependent phospholipid binding proteins that are preferentially located on the cytosolic face of the plasma membrane. Annexin I has a molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ANAX1; Annexin 1 (p35) (Lipocortin 1); Annexin A1; Annexin Ac2-26; Annexin I; annexin I (lipocortin I); Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; lipocortin 1; Lipocortin I; p35; Phospholipase A2 inhibitory protein; RP11-71A24.1

Gene Aliases: Anx-1; Anx-A1; ANX1; ANXA1; C430014K04Rik; Lpc-1; LPC1; p35

UniProt ID: (Human) P04083, (Mouse) P10107, (Rat) P07150

Entrez Gene ID: (Human) 301, (Mouse) 16952, (Rat) 25380

Molecular Function: calcium-binding protein

DNA strand renaturation neutrophil homeostasis adaptive immune response monocyte chemotaxis regulation of leukocyte migration phagocytosis inflammatory response signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger regulation of cell shape response to X-ray myoblast migration involved in skeletal muscle regeneration peptide cross-linking insulin secretion keratinocyte differentiation prostate gland development endocrine pancreas development positive regulation of vesicle fusion positive regulation of prostaglandin biosynthetic process actin cytoskeleton reorganization response to estradiol DNA duplex unwinding regulation of interleukin-1 production positive regulation of interleukin-2 production positive regulation of neutrophil apoptotic process DNA rewinding gliogenesis positive regulation of T cell proliferation response to drug negative regulation of apoptotic process response to peptide hormone estrous cycle innate immune response positive regulation of T-helper 1 cell differentiation negative regulation of T-helper 2 cell differentiation negative regulation of exocytosis alpha-beta T cell differentiation regulation of hormone secretion arachidonic acid secretion regulation of inflammatory response cellular response to hydrogen peroxide hepatocyte differentiation prolactin secretion response to interleukin-1 cellular response to glucocorticoid stimulus granulocyte chemotaxis positive regulation of wound healing neutrophil clearance positive regulation of G1/S transition of mitotic cell cycle negative regulation of phospholipase A2 activity negative regulation of interleukin-8 secretion regulation of cell proliferation positive regulation of apoptotic process negative regulation of protein secretion response to hormone response to organic cyclic compound response to corticosteroid response to glucocorticoid

Process(es)

single-stranded DNA binding single-stranded RNA binding helicase activity receptor binding structural molecule activity calcium ion binding protein binding phospholipid binding calcium-dependent phospholipid binding phospholipase A2 inhibitor activity protein binding, bridging double-stranded DNA-dependent ATPase activity annealing helicase activity protein homodimerization activity calcium-dependent protein binding cadherin binding involved in cell-cell adhesion phospholipase inhibitor activity