Invitrogen

Phospho-Annexin A2 (Ser26) Polyclonal Antibody

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Phospho-Annexin A2 (Ser26) Antibody in Western Blot (WB) — 2 FIGURE PKC phosphorylates ANXA2 on Ser25 side chain. (a) Purified recombinant GST, GST-ANXA2, and GST-TIMAP proteins were incubated with or without active PKCalpha for 30 min. Amount of loaded proteins were verified by SDS-PAGE followed by Coomassie staining. Phosphorylation of proteins was analyzed by western blot using anti-phosphoSer PKC substrate and phospho-Ser25 annexin A2-specific antibodies. (b) Pull down of endothelial cell lysate was made using GST, GST-tagged TIMAP wild type, S331D or S331D recombinant full-length TIMAP proteins as baits. Samples were tested for ANXA2 in western blot experiment. (c) Total cell lysates of control, nonsilencing RNA and PKC-specific siRNA transfected HPAEC treated with PMA (1 muM for 30 min) or PMA (1 muM for 30 min) after a pretreatment with Go6983 (1 muM for 30 min) were analyzed by western blot using PKCalpha, actin, ANXA2, and phospho-Ser25 ANXA2-specific antibodies. (d) Western blot analysis of control, PMA (1 muM, 30 min), PMA (1 muM, 30 min) with Go6983 pretreatment (1 muM, 30 min), okadaic acid (5 nM, 30 min), tautomycetin (1 muM, 30 min), nonsiRNA, or TIMAP-specific siRNA-treated BPAEC cells are shown. Phospho-Ser25 ANXA2 level was normalized to ANXA2 protein level. Densitometric analysis of western blots are shown as mean +- SD ( n = 3). Statistical analysis was done with ANOVA and marked by asterisks; *** p < .001

Phospho-Annexin A2 (Ser26) Antibody in Western Blot (WB) — Figure 5 Phosphorylation of AnxA2 in breast cancer cells. ( A ) The expression of pAnxA2-S25, pAnxA2-Y23, and AnxA2 were analyzed in non-tumorigenic mammary epithelial cells and TNBC cells by immunoblot analysis. The membrane was stripped and reprobed with anti-beta-actin antibody for loading control. ( B ) Bar chart showing the densitometric analysis of pAnxA2-S25, pAnxA2-Y23 and AnxA2 bands of the immunoblot of panel A. Intensity of each bands were normalized by loading control. Each bar represents the mean +- SE of three independent experiments. ( C ) The HCC1395 cells were transfected with a plasmid vector expressing GFP alone, AnxA2-WT-GFP, and AnxA2-Y23F-GFP. The expression of pAnxA2-Y23, AnxA2 and GFP were analyzed by immunoblot analysis. The membrane was stripped and reprobed with anti-beta-actin antibody for loading control. ( D ) The HCC1395 transfected cells (6 x 10 5 ) were plated in 100mm Petri dish for overnight and then switched to serum free medium. After 24 h, the AnxA2-GFP complex was immunoprecipitated from the medium using anti-GFP antibody (Catalog no. DSHB-GFP-12A6; DSHB) and expression of AnxA2 secretion was analyzed by anti-AnxA2 antibody using immunoblot analysis. Uncropped Western Blots of ( A , C , D ) are available in Figure S3 .

Product Details

PA5-37474

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human
Published species	Bovine, Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide sequence around phosphorylation site of Serine 26 (Y-G-S(p)-V-K) derived from Human ANXA2. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2554083

Product Specific Information

A suggested positive control for Western blot is HepG2 or JK cells.
The homology between synthetic peptide sequence and Mouse/Rat is 100%.

Target Information

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dephosphorylation of annexin A2 by protein phosphatase 1 regulates endothelial cell barrier.

IUBMB life

Király N,Thalwieser Z,Fonódi M,Csortos C,Boratkó A

Published figure using Phospho-Annexin A2 (Ser26) polyclonal antibody (Product # PA5-37474) in Western Blot

Fri Oct 01 00:00:00 EDT 2021

Dephosphorylation of annexin A2 by protein phosphatase 1 regulates endothelial cell barrier.

IUBMB life

Király N,Thalwieser Z,Fonódi M,Csortos C,Boratkó A

Published figure using Phospho-Annexin A2 (Ser26) polyclonal antibody (Product # PA5-37474) in Western Blot

Fri Oct 01 00:00:00 EDT 2021

Dephosphorylation of annexin A2 by protein phosphatase 1 regulates endothelial cell barrier.

IUBMB life

Király N,Thalwieser Z,Fonódi M,Csortos C,Boratkó A

Published figure using Phospho-Annexin A2 (Ser26) polyclonal antibody (Product # PA5-37474) in Western Blot

Fri Oct 01 00:00:00 EDT 2021

Clinical Significance of Annexin A2 Expression in Breast Cancer Patients.

Cancers

Gibbs LD,Mansheim K,Maji S,Nandy R,Lewis CM,Vishwanatha JK,Chaudhary P

Published figure using Phospho-Annexin A2 (Ser26) polyclonal antibody (Product # PA5-37474) in Western Blot

Tue Dec 22 00:00:00 EST 2020

Clinical Significance of Annexin A2 Expression in Breast Cancer Patients.

Cancers

Gibbs LD,Mansheim K,Maji S,Nandy R,Lewis CM,Vishwanatha JK,Chaudhary P

Published figure using Phospho-Annexin A2 (Ser26) polyclonal antibody (Product # PA5-37474) in Western Blot

Tue Dec 22 00:00:00 EST 2020

Bioinformatics

Protein Aliases: Annexin A2; Annexin II; Annexin-2; Calpactin I heavy chain; calpactin I heavy polypeptide; Calpactin-1 heavy chain; chromobindin 8; Chromobindin-8; epididymis secretory protein Li 270; Lipocortin II; p36; PAP-IV; Placental anticoagulant protein IV; Protein I

Gene Aliases: ANX2; ANX2L4; ANXA2; CAL1H; HEL-S-270; LIP2; LPC2; LPC2D; P36; PAP-IV

UniProt ID: (Human) P07355

Entrez Gene ID: (Human) 302

Molecular Function: calcium-binding protein

angiogenesis membrane raft assembly positive regulation of protein phosphorylation negative regulation of receptor internalization membrane budding body fluid secretion collagen fibril organization positive regulation of vesicle fusion negative regulation of low-density lipoprotein particle receptor catabolic process osteoclast development fibrinolysis negative regulation of catalytic activity positive regulation of fibroblast proliferation positive regulation of binding protein heterotetramerization protein targeting to plasma membrane response to thyroid hormone cell-cell adhesion negative regulation of receptor binding positive regulation of receptor activity

Process(es)

protease binding calcium ion binding protein binding calcium-dependent phospholipid binding phosphatidylinositol-4,5-bisphosphate binding cytoskeletal protein binding Rab GTPase binding phospholipase A2 inhibitor activity receptor activator activity S100 protein binding poly(A) RNA binding calcium-dependent protein binding cadherin binding involved in cell-cell adhesion