Immunofluorescent analysis of Phospho-Aurora A pThr288 in mitotic HeLa cells during metaphase anaphase using a Phospho-Aurora A pThr288 monoclonal antibody (Product # MA5-14904) (red) and a Phospho-Histone H3 (Ser10) monoclonal antibody (green). DNA is labeled using a fluorescent blue dye.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues around pThr288 of human Aurora A|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
It is not recommended to aliquot this antibody.
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A FRET biosensor reveals spatiotemporal activation and functions of aurora kinase A in living cells.
MA5-14904 was used in western blot develop and characterize a Forster's resonance energy transfer-based aurora kinase A biosensor
|Bertolin G,Sizaire F,Herbomel G,Reboutier D,Prigent C,Tramier M||Nature communications (7:null)||2016|