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Western blot analysis of Phospho-Aurora B pThr236+Aurora C pThr202 using Phospho-Aurora B pThr236+Aurora C pThr202 polyclonal antibody (Product # PA5-36030) at a dilution of 1:500. Lane 1: HEK293T whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: H9C2 whole cell lysate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human AURKB/C around the phosphorylation site of Threonine 236/202|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 35 and 39 kDa.
Purity is >95% by SDS-PAGE.
Aurora kinases belong to a highly conserved family of mitotic serine/threonine kinases with three members identified among mammals: Aurora A, B, and C. Studies on the temporal expression pattern and subcellular localization of Aurora kinases in mitotic cells suggest an association with mitotic structure. Aurora kinase functional influences span from G2 phase to cytokinesis and may be involved in key cell cycle events such as centrosome duplication, chromosome bi-orientation and segregation, cleavage furrow positioning, and ingression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AIE2; AIK2; AIK3; AIM-1; AIM1; AIRK2; AIRK3; ARK-2; ARK-3; ARK2; ARK3; AURKB; AURKC; aurora 1; aurora 3; aurora B; aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; aurora kinase C; aurora- and IPL1-like midbody-associated protein 1; aurora-1; aurora-B; Aurora-related kinase 2; Aurora-related kinase 3; aurora/IPL1-related kinase 2; aurora/IPL1-related kinase 3; aurora/Ipl1/Eg2 protein 1; aurora/IPL1/EG2 protein 2; epididymis secretory protein Li 90; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; serine/threonine kinase 13 (aurora/IPL-like); serine/threonine kinase 13 (aurora/IPL1-like); serine/threonine kinase 5; serine/threonine-protein kinase 12; serine/threonine-protein kinase 13; serine/threonine-protein kinase 5; serine/threonine-protein kinase aurora-B; serine/threonine-protein kinase aurora-C; STK-1; STK12; STK13; STK5
AIE1; AIE2; AIK2; AIK3; AIM-1; AIM1; AIRK2; AIRK3; AL022959; ARK-2; ARK-3; ARK2; ARK3; AurB; AurC; AURKB; aurkb-sv1; aurkb-sv2; AURKC; aurora-C; HEL-S-90; IAK3; IPL1; PPP1R48; SPGF5; STK-1; STK1; STK12; STK13; STK5