|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human B-Myb around the phosphorylation site of Serine 577|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 80 and 100 kDa.
Purity is >95% by SDS-PAGE.
c-Myb is the cellular homolog of the leukemogenic avian retroviral protein v-Myc. c-Myb is expressed predominantly in immature and rapidly dividing hematopoietic cells, and cellular levels of c-Myb substantially decreases as cells reach terminal differentiation. B-Myb is expressed in a wide variety of proliferating cells, with levels accumulating during the G1 to S phase transition. A-Myb is expressed at specific times in reproductive tissues, some neural cells, and a subset of normal and neoplastic B lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.