Immunofluorescent analysis of Phospho-BCL-2 pSer70 in untreated SH-SY5Y cells using a Phospho-BCL-2 pSer70 monoclonal antibody (Product # MA5-15046) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding serine 70 of human Bcl-2|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with nonphosphorylated Bcl-2 at endogenous levels or with other Bcl-2 family members.
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
NF-¿B RNAi decreases the Bax/Bcl-2 ratio and inhibits TNF-¿-induced apoptosis in human alveolar epithelial cells.
MA5-15046 was used in western blot to study the role of a lowered Bax/Bcl-2 ratio in the mechanism by which NFkB knockdown inhibits the induction of human alveolar epithelial cell apoptosis induced by TNF-alpha
|Li L,Wu W,Huang W,Hu G,Yuan W,Li W||Inflammation research : official journal of the European Histamine Research Society ... [et al.] (62:387)||2013|