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Invitrogen

Phospho-BTK/ITK (Tyr551, Tyr511) Recombinant Mouse Monoclonal Antibody (M4G3LN)

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Product Details

740047M

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200
-

Flow Cytometry (Flow)

0.125 - 0.25 µg/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

M4G3LN

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Amount

100 µg

Purification

Protein A/G

Storage buffer

PBS, pH 7.2-7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3679158

Product Specific Information

Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, lot-to-lot consistency, and broader immunoreactivity to diverse targets due to a larger rabbit immune repertoire.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Target Information

In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: agammaglobulinaemia tyrosine kinase; B-cell progenitor kinase; Bruton agammaglobulinemia tyrosine kinase; Bruton's tyrosine kinase; dominant-negative kinase-deficient Brutons tyrosine kinase; frameshift mutation results in truncated protein; homolog of mouse T-cell itk/tsk; IL-2-inducible T-cell kinase; interleukin-2-inducible T-cell kinase; kinase EMT; T-cell-specific kinase; tyrosine-protein kinase LYK; unnamed protein product

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Gene Aliases: AGMX1; AT; ATK; BPK; EMT; IGHD3; IMD1; LPFS1; LYK; PSCTK1; PSCTK2; XLA

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UniProt ID: (Human) Q06187, (Human) Q08881

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Entrez Gene ID: (Human) 695, (Human) 3702

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Function(s)
protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding phospholipase activator activity identical protein binding phospholipase binding
Process(es)
neutrophil homeostasis positive regulation of type III hypersensitivity positive regulation of type I hypersensitivity negative regulation of cytokine production adaptive immune response histamine secretion by mast cell positive regulation of immunoglobulin production regulation of B cell cytokine production MyD88-dependent toll-like receptor signaling pathway regulation of B cell apoptotic process mesoderm development peptidyl-tyrosine phosphorylation calcium-mediated signaling proteoglycan catabolic process positive regulation of B cell proliferation response to lipopolysaccharide negative regulation of interleukin-10 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production cellular response to reactive oxygen species intracellular signal transduction Fc-epsilon receptor signaling pathway B cell activation positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response positive regulation of B cell differentiation positive regulation of phagocytosis T cell receptor signaling pathway B cell receptor signaling pathway regulation of B cell activation negative regulation of B cell activation monocyte proliferation negative regulation of leukocyte proliferation cellular response to molecule of fungal origin apoptotic signaling pathway positive regulation of cGAS/STING signaling pathway positive regulation of interleukin-17A production positive regulation of NLRP3 inflammasome complex assembly positive regulation of synoviocyte proliferation eosinophil homeostasis positive regulation of cytokine production NK T cell differentiation cellular defense response signal transduction T cell activation gamma-delta T cell activation
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