Western blot analysis of lysates prepared from EB1 cells using rabbit anti-BTK [pY223] antibody (Cat. no. 44-1350G). Cells left untreated (lane 1) or treated with hydrogen peroxide (lanes 2-5) were resolved on a 10 percent polyacrylamide gel and transferred to PVDF. The membrane was treated with either no peptide (lane 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (lane 3), a generic phosphotyrosine containing peptide (lane 4), or the phosphopeptide immunogen (lane 5). Prior to incubation with the primary antibody, lane 6 was treated with Lambda phosphatase.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human BTK that contains tyrosine 223.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Bruton and quote;s tyrosine kinase (BTK) was initially identified as a member of the src family for protein-tyrosine kinases that was involved in X-linked a-gamma-globulinaemia, and has since been shown to involved in a number of signaling pathways in hemapoietic lineage. It has recently been shown to interact with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9. The TLRs are critical molecules in both the innate and adaptive immunity and can recognize diverse microbial pathogens. BTK has also been shown to interact with key proteins involved in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting that BTK is involved in lipopolysaccharide signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.