Western blot analysis of phospho Bcr (Y276) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 276 and 2) an unphosphorylated tyrosine at position 276, using a Phospho-Bcr/Abl pTyr276 polyclonal antibody (Product # PA5-23329) at 0.01 ug/ml.
|Tested species reactivity||Human, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (265 HKLGGGQYGEV(Y)EGV 279) of Phospho-Bcr/Abl pTyr276 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.01-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Bcr, receptor tyrosine kinase (also known as breakpoint cluster region or C-abl oncogene 1) is an intracellular protein belonging to the protein kinase superfamily and Tyr protein kinase family. It is a proto-oncogene with a SH2 domain, a SH3 domain and a protein kinase domain. Purified Bcr is known to contain autophosphorylation activity and transphosphorylation activity for several substrates. The function of this protein includes promoting the exchange of RAC or CDC42-bound GDP by GTP thereby activating them and hence acting as a GTPase activating protein. Moreover, PCAF and BCR signalling are essential for apoptotic cell death (PCAF and BCR signalling control cooperatively pre-mature B cell apoptosis via both depletions of DFFA and BIRC2). Ubiquitous expression of Bcr is seen in most tissues. Bcr with Abl1, in reciprocal translocation between chromosomes 22 and 9, produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.