Western blot analysis of Phospho-Beclin1 pSer295 in flag-beclin-1 wild-type immunoprecipitates expressed in 293T cells using a Phospho-Beclin1 pSer295 polyclonal antibody (Product # PA5-35394) showing specific immunolabeling of beclin-1 when phosphorylated at S295 (WT). This immunolabeling is eliminated in the beclin-1 alanine substitution mutant (S295A).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 of human BECN1 conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 0.1mg/ml BSA, 50% glycerol, 0.15M NaCl|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:250|
This antibody is predicted to react with bovine, canine, chicken, mouse, non-human primate, sheep, Xenopus and zebrafish based on 100% sequence homology.
In Western blot, additional bands may be observed when using non-immunoprecipitated lysates.
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.