Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
b-Arrestin [pS412] phosphospecific antibody. PC12 cell extracts were either not treated (1, 3) or treated (2, 4) with PP2A phosphatase (Cat. no. PHZ1044), then incubated with b-arrestin-1 pan (1, 2), or [pS412] antibody (3, 4). Data demonstrate antibody specificity.
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthesized phosphopeptide derived from the region of rat beta-arrestin-1 that contains serine 412.|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold maolecule, beta Arrestin 1 is essential for mitogenic signalling and mediates agonist-dependent desensitization and internalization of Gprotein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosporylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1&2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1&2 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Human||Not Cited||MEK1 binds directly to betaarrestin1, influencing both its phosphorylation by ERK and the timing of its isoprenaline-stimulated internalization.||Meng D,Lynch MJ,Huston E,Beyermann M,Eichhorst J,Adams DR,Klussmann E,Klusmann E,Houslay MD,Baillie GS||The Journal of biological chemistry (284:11425)||2009|
ARR1; ARRB1; arrestin beta-1; beta-arrestin-1; beta-arrestin1
1200006I17Rik; Arrb1; AW208571; BARRES; G430100A01Rik