|Tested species reactivity||Human, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of rat BimEL that contains serine 65 (serine 69 in the human sequence). The sequence is conserved in human and mouse.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Bcl-2 (B-cell lymphoma) and close homologues promote cell survival while many of its relatives lead to apoptotic cell death. The Bcl-2 homology 3 (BH3) domain is a potent death domain, and a novel BH3 domain containing protein, termed Bim or BOD (Bcl-2-related Ovarian Death Gene), has recently been cloned. Bim possesses a hydrophobic C-terminus and is localized to the intracytoplasmic membranes. Bcl-2 binds to Bim and modulates its death function, while Bcl-2 mutants are unable to do either.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
||betaTrCP- and Rsk1/2-mediated degradation of BimEL inhibits apoptosis.||Dehan E,Bassermann F,Guardavaccaro D,Vasiliver-Shamis G,Cohen M,Lowes KN,Dustin M,Huang DC,Taunton J,Pagano M||Molecular cell (33:109)||2009|
|Mouse||Not Cited||betaTrCP- and Rsk1/2-mediated degradation of BimEL inhibits apoptosis.||Dehan E,Bassermann F,Guardavaccaro D,Vasiliver-Shamis G,Cohen M,Lowes KN,Dustin M,Huang DC,Taunton J,Pagano M||Molecular cell (33:109)||2009|