Western blot analysis of extracts from Jurkat cells, untreated or treated with TPA (40 nM) and calcium ionophore A23187 (2µM) for 15 minutes, and L-phosphatase plus calf intestinal phosphatase (CIP) as indicated, using CARD11 (pS652) Polyclonal (top) and a CARD11 antibody (bottom).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic phosphopeptide corresponding to a region surrounding Ser652 of human CARD11 protein|
|Storage buffer||0.01M HEPES, pH 7.5, with 50% glycerol, 100µg/ml BSA, 0.15M NaCl|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.
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