Western blot analysis of Phospho-CD32B/FCGR2B pTyr292 using Phospho-CD32B/FCGR2B pTyr292 polyclonal antibody (Product # PA5-36794) at a dilution of 1:500. Lane 1: MCF-7 cell lysate treated with pervanadate (25ng/ml, 72h), Lane 2: Mouse liver tissue lysate, Lane 3: PC12 cell lysate treated with pervanadate (25ng/ml, 72h).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human CD32-B around the phosphorylation site of Tyrosine 292|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 40 kDa.
Purity is >95% by SDS-PAGE.
CD32 (also designated Fc gamma RII) is a low affinity receptor for the Fc fragment of aggregated IgG. CD32 is responsible for the clearance of immunocomplexes by macrophages and also plays an important role in the regulation of antibody production by B cells. IgG can noncooperatively bind either one or two highly glycosylated CD32 molecules, and this binding delivers.3 a negative signal for B cells. CD32 exists as several isoforms that are produced by alternative splicing of three distinct genes, A, B, and C. These isoforms are designated FcgRIIA, FcgRIIB1, FcgRIIB3, and FcgRIIC. All isoforms are present on monocytes, placental trophoblasts and endothelial cells. In addition, the FcgRIIB forms are present on B lymphocytes, and the FcgRIIA and FcgRIIC forms are found on neutrophils.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.