Invitrogen

Phospho-CDK1 (Thr14, Tyr15) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

12 References

View all (90) CDK1 antibodies

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Phospho-CDK1 (Thr14, Tyr15) Antibody in Western Blot (WB)

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Phospho-CDK1 (Thr14, Tyr15) Antibody (44-686G) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (1), HeLa treated for 20 hr with 3mM of Hydroxy Urea (2), HeLa Serum Starved (3), HeLa Serum Starved overnight following by serum release (4), HeLa treated with UV for 40 min (5), Jurkat (6), Jurkat Serum Starved (7), Jurkat Serum Starved overnight following by serum release (8), Jurkat treated with UV for 40 min (9) and U2OS ... View More

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Product Details

44-686G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 12 publications 12 publications

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat, Xenopus, Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antibody was produced against a synthetic phosphopeptide derived from the region of the human cyclin-dependent kinase that contains threonine 14 and tyrosine 15.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533722

Target Information

CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mechanisms of synergistic suppression of ALK-positive lung cancer cell growth by the combination of ALK and SHP2 inhibitors.

Scientific reports

Berry MA,Bland AR,Ashton JC

44-686G was used in Western Blotting to investigate whether the combination of a SHP2 inhibitor (SHP099) with alectinib would synergistically suppress the growth of ALK-positive lung cancer cells.

Tue Jun 20 00:00:00 EDT 2023

Mre11 exonuclease activity promotes irreversible mitotic progression under replication stress.

Life science alliance

Hashimoto Y,Tanaka H

44-686G was used in Western Blot to suggest that Mre11 exonuclease activity facilitates the processing of stalled replication forks upon mitotic entry, which attenuates the inhibitory pathways of mitotic CDK activation, leading to irreversible mitotic progression and replisome disassembly.

Wed Jun 01 00:00:00 EDT 2022

Mre11 exonuclease activity promotes irreversible mitotic progression under replication stress.

Life science alliance

Hashimoto Y,Tanaka H

Wed Jun 01 00:00:00 EDT 2022

Multi-omics Profiling Shows BAP1 Loss Is Associated with Upregulated Cell Adhesion Molecules in Uveal Melanoma.

Molecular cancer research : MCR

Baqai U,Purwin TJ,Bechtel N,Chua V,Han A,Hartsough EJ,Kuznetsoff JN,Harbour JW,Aplin AE

44-686G was used in Western Blotting to demonstrate that BAP1 regulates the expression of CAMs which may regulate metastatic traits.

Fri Aug 05 00:00:00 EDT 2022

Metabolite and thymocyte development defects in ADA-SCID mice receiving enzyme replacement therapy.

Scientific reports

Moretti FA,Giardino G,Attenborough TCH,Gkazi AS,Margetts BK,la Marca G,Fairbanks L,Crompton T,Gaspar HB

Published figure using Phospho-CDK1 (Thr14, Tyr15) polyclonal antibody (Product # 44-686G) in Western Blot

Wed Dec 01 00:00:00 EST 2021

Ongoing replication forks delay the nuclear envelope breakdown upon mitotic entry.

The Journal of biological chemistry

Hashimoto Y,Tanaka H

44-686G was used in Western Blotting to investigate the consequences that arise when stalled forks are released simultaneously with the induction of mitosis.

Fri Aug 27 00:00:00 EDT 2021

Effects of Oncogenic Gα_q and Gα₁₁ Inhibition by FR900359 in Uveal Melanoma.

Molecular cancer research : MCR

Lapadula D,Farias E,Randolph CE,Purwin TJ,McGrath D,Charpentier TH,Zhang L,Wu S,Terai M,Sato T,Tall GG,Zhou N,Wedegaertner PB,Aplin AE,Aguirre-Ghiso J,Benovic JL

44-686G was used in Western Blotting to demonstrate that FR inhibits oncogenic Gαq/11-Q209 protein in vitro and in uveal melanoma (UM) cells, promoting cell cycle arrest and eventual cell death, while also preventing the colonization of UM cells in 3D culture.

Mon Apr 01 00:00:00 EDT 2019

4-Hydroxybenzoic acid derivatives as HDAC6-specific inhibitors modulating microtubular structure and HSP90α chaperone activity against prostate cancer.

Biochemical pharmacology

Seidel C,Schnekenburger M,Mazumder A,Teiten MH,Kirsch G,Dicato M,Diederich M

44-686G was used in western blot to characterize modulation of microtubular structure and HSP90alpha chaperone activity against prostate cancer by 4-hydroxybenzoic acid derivatives as HDAC6-specific inhibitors

Fri Jan 01 00:00:00 EST 2016

A novel coumarin-quinone derivative SV37 inhibits CDC25 phosphatases, impairs proliferation, and induces cell death.

Molecular carcinogenesis

Bana E,Sibille E,Valente S,Cerella C,Chaimbault P,Kirsch G,Dicato M,Diederich M,Bagrel D

44-686G was used in western blot to characterize new inhibitors of CDC25.

Sun Mar 01 00:00:00 EST 2015

A novel coumarin-quinone derivative SV37 inhibits CDC25 phosphatases, impairs proliferation, and induces cell death.

Molecular carcinogenesis

Bana E,Sibille E,Valente S,Cerella C,Chaimbault P,Kirsch G,Dicato M,Diederich M,Bagrel D

44-686G was used in western blot to characterize new inhibitors of CDC25.

Sun Mar 01 00:00:00 EST 2015

Upregulation of p21 activates the intrinsic apoptotic pathway in β-cells.

American journal of physiology. Endocrinology and metabolism

Hernandez AM,Colvin ES,Chen YC,Geiss SL,Eller LE,Fueger PT

44-686G was used in western blot to study the role of p21 in beta cell apoptosis

Sat Jun 15 00:00:00 EDT 2013

Multisite phosphoregulation of Cdc25 activity refines the mitotic entrance and exit switches.

Proceedings of the National Academy of Sciences of the United States of America

Lu LX,Domingo-Sananes MR,Huzarska M,Novak B,Gould KL

Tue Jun 19 00:00:00 EDT 2012

The extracellular signal-regulated kinase-mitogen-activated protein kinase pathway phosphorylates and targets Cdc25A for SCF beta-TrCP-dependent degradation for cell cycle arrest.

Molecular biology of the cell

Isoda M,Kanemori Y,Nakajo N,Uchida S,Yamashita K,Ueno H,Sagata N

44-686G was used in Western Blotting to demonstrate that strong ERK activation can phosphorylate and target Cdc25A for SCF(beta-TrCP)-dependent degradation.

Wed Apr 01 00:00:00 EDT 2009

Dynamic expression of the RNA-binding protein Sam68 during mouse pre-implantation development.

Gene expression patterns : GEP

Paronetto MP,Bianchi E,Geremia R,Sette C

44-686G was used in western blot to assess the expression and localization of Sam68 during early mouse embryogenesis

Thu May 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: ABL1; CDK1; cell cycle controller CDC2; cell cycle p34 CDC2 kinase protein; Cell division control protein 2 homolog; cell division cycle 2 homolog A; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; cell division cycle control protein 2a; Cell division protein kinase 1; Cyclin-dependent kinase 1; DKFZp686L20222; MGC111195; MGC53829; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase

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Gene Aliases: CDC2; CDC28A; Cdc2a; CDK1; CDKN1; p34; P34CDC2

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UniProt ID: (Human) P06493, (Rat) P39951, (Mouse) P11440

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Entrez Gene ID: (Human) 983, (Rat) 54237, (Mouse) 12534

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Function(s)

protein kinase activity protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity cyclin binding Hsp70 protein binding histone kinase activity kinase activity nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

Process(es)

G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle activation of MAPK activity microtubule cytoskeleton organization DNA replication DNA repair protein complex assembly apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest mitotic nuclear division mitotic nuclear envelope disassembly mitotic G2 DNA damage checkpoint centrosome cycle pronuclear fusion cell aging response to toxic substance positive regulation of gene expression regulation of Schwann cell differentiation response to organic cyclic compound response to amine response to activity cell migration histone phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation chromosome condensation epithelial cell differentiation organ regeneration anaphase-promoting complex-dependent catabolic process positive regulation of protein import into nucleus, translocation protein localization to kinetochore response to drug protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process response to ethanol positive regulation of DNA replication positive regulation of mitotic cell cycle regulation of embryonic development response to cadmium ion response to copper ion response to axon injury cell division negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle ventricular cardiac muscle cell development positive regulation of cardiac muscle cell proliferation cellular response to hydrogen peroxide Golgi disassembly positive regulation of protein localization to nucleus mitotic cell cycle protein phosphorylation response to organonitrogen compound response to hydrogen peroxide cell cycle phosphorylation

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Phospho-CDK1 (Thr14, Tyr15) Polyclonal Antibody

Phospho-CDK1 (Thr14, Tyr15) Antibody (44-686G) in WB

Product Details

44-686G

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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